University of Minnesota

Other

Colorado State University Research Foundation

Lawrence Philip Wackett

Michael J Sadowsky

Mervyn L De Souza

Jeremy S Minshull

Jennifer L Seffernick

Kenneth F Reardon

Alptekin Aksan

Eduardo Reategui

Lisa Kasinkas

Kelly Aukema

Carroll P Vance

Deborah A Samac

Raphael T Mandelbaum

The present invention relates to engineered cyanuric acid hydrolase enzymes that are resistant to hypochlorite and compositions and devices comprising such enzymes. The present invention also relates to methods of using these enzymes, compositions and devices for the treatment of a liquid, such as water.

Hypochlorite resistant cyanuric acid hydrolases and methods of use thereof

The present invention relates to compositions for encapsulation of biomaterials in a silica-matrix. The present invention includes a composition for formation of a silica-matrix encapsulated biomaterial. The composition includes a reactive silicon compound and a biomaterial with a catalytic activity. When encapsulated in the silica-matrix, the biomaterial at least partially retains its catalytic activity. The present invention also relates to methods of making silica-matrix encapsulated biomaterials, and to methods of using silica-matrix encapsulated biomaterials, including methods of treating water or gas using the silica-matrix encapsulated biomaterials.

Silica encapsulated biomaterials

The present invention relates to stable cyanuric acid hydrolase enzymes, compositions, and devices for use in the treatment of a liquid, such as water. The present invention also relates to methods of using these enzymes, compositions and devices for the treatment of a liquid, such as water.

Methods, compositions and devices utilizing structurally stable cyanuric acid hydrolase

Enzymatic biosensors and methods of producing distal tips for biosensor transducers for use in detecting one or more analytes selected from organic compounds susceptible to dehalogenation, organic compounds susceptible to oxygenation, organic compounds susceptible to deamination, organosulfate compounds susceptible to hydrolysis, and organophosphate compounds susceptible to hydrolysis are disclosed herein, as well as biosensor arrays, methods of detecting and quantifying analytes within a mixture, and devices and methods for delivering reagents to enzymes disposed within the distal tip of a biosensor.

Distal tip of biosensor transducer comprising enzyme for deamination

This invention relates to the identification of homologs of atrazine chlorohydrolase and the use of these homologs to degrade s-triazine-containing compounds. In particular, this invention includes the identification of homologs of atrazine chlorohydrolase encoded by a DNA fragment having at least 95% homology to the sequence from the nucleic acid sequence beginning at position 236 and ending at position 1655 of SEQ ID NO:1, where the DNA fragment is capable of hybridizing under stringent conditions to SEQ ID NO:1 and has altered catalytic activity as compared with wild-type atrazine chlorohydrolase.

DNA molecules and protein displaying improved triazine compound degrading ability

The present invention provides plants that dehalogenate at least one s-triazine. The plants can include an exogenous coding region that imparts the ability to degrade at least one s-triazine. The present invention further provides methods for degrading, more preferably detoxifying, at least one s-triazine with an s-triazine degrading plant.

Transgenic plants expressing bacterial atrazine degrading gene AtzA

An isolated and purified DNA molecule, and an isolated and purified protein, that are involved in the degradation of s-triazine compounds (e.g., atrazine) are provided. A method for the purification of this protein is also provided.

Isolated and purified DNA molecule and protein for the degradation of triazine compounds

A method for degradation of s-triazine compounds, including atrazine, which utilizes an atrazine degrading Pseudomonas (ADP) bacterium and its enzymes is provided. ADP was isolated from atrazine-contaminated soil and is phylogenetically close to P. aeruginosa and P. citronellolis. When ADP is cultivated on atrazine medium, it produces enzymes that can completely degrade atrazine to yield CO.sub.2 from ring carbons.

Growing community of inventors

Saint Paul, MN, United States of America

Lawrence Philip Wackett