Purdue Research Foundation

University of Minnesota

Other

Yale University

Laurie Louise Parker

Andrew Michael Lipchik

Scott Charles Bolton

Wei Cui

Vincent Jo Davisson

Benjamin Turk

Tzu-Yi Yang

Joseph Maria Kumar Irudayarsaj

Laura Marholz

Nur Pradani Damayanti

Robert John Schuster

Minervo Perez

The invention provides kinase substrates and methods comprising their use.

Universal kinase substrates and methods of use thereof

The invention provides cellular assays for detecting the activity of one or more kinase from multiple conditions simultaneously, by encoding biochemically identical substrates with isotope labels that enable them to be distinguished in pooled samples by mass spectrometry.

Kinase activity detection methods

Kinase substrates and methods of use thereof

Disclosed are compositions and methods for measuring tyrosine kinase activity using degradation resistant biosensors comprising a reporter sequence, a target sequence, and a transduction sequence. In certain embodiments, the invention includes a biosensor comprising a peptide comprising a reporter sequence, i.e., an amino acid sequence including a tyrosine residue that can be phosphorylated by a tyrosine kinase. In certain embodiments, a biosensor comprises a reporter sequence configured to resist degradation from proteases, a targeting sequence coupled to the reporter sequence and configured to bind to a protein interaction domain of a kinase, and a transduction sequence coupled to the targeting sequence.

Degradation resistant peptide based biosensors

Substrates for FLT3 kinase and uses thereof

The present disclosure illustrates new synthetic ALK-specific biosensors used to measure the activity or ALK, and methods of using the synthetic ALK-specific biosensors in assays, including high throughput assays.

Tyrosine kinase biosensors and methods of use

Disclosed are compositions and methods for measuring tyrosine kinase activity.

Disclosed are methods of detecting enzymatic activity on a fluorophore-labeled substrate using by monitoring the fluorescence lifetime of the fluorophore.

Methods for detecting enzyme activity using fluorescence lifetime imaging

The claims describe a process for designing and validating peptide-based substrates for a specified kinase. The process uses a rational approach to design peptides with a specific amino acid that is phosphorylated by a target tyrosine kinase, and upon phosphorylation shows an increased affinity for a lanthanide ion that results in an enhanced fluorescent signal. The process uses kinase specific input data sets, encodes the input into positional scoring matrices, analyses the positional scoring matrices in order to generate a candidate list of peptide sequences, compares the peptide sequences in the candidate list, substitutes residues at non-selective positions in selections from the candidate list with terbium binding residues, and then screens the substituted selections with different kinases in vitro.

Growing community of inventors

Minneapolis, MN, United States of America

Laurie Louise Parker