Biomerieux, Inc.

Biomerieux Sa

Biofire Diagnostics, LLC

Biofire Defense, LLC

Commissariat a L'energie Atomique Et Aux Energies Alternatives

Commissariat À L'énergie Atomique Et Aux Énergies Alternatives

Laurent Mesta

Ali Laayoun

David E Jones

Kirk M Ririe

Aaron D Wernerehl

Agnès Dupont-Filliard

Christopher Paul Pasko

Erik Wong Huynh

Carole Vachon

Andrew C Hatch

Alain Laurent

Jean-Claude Boulain

Frédéric Ducancel

Alain Troesch

Janie Dassa

Bruno H Muller

Systems, methods, and apparatuses are provided for self-contained nucleic acid preparation, amplification, and analysis.

Assay devices and methods of use thereof

The present invention pertains to a mutated T7 RNA polymerase and its use, the T7 RNA polymerase being mutated at position 744, the glutamine (Q) being replaced by an amino acid selected from arginine (Q744R), leucine (Q744L) or proline (Q744P).

Mutated T7 RNA Polymerases

A method of transcriptional amplification includes: (a) obtaining a mixture by combining (i) a biological sample comprising nucleic acids, (ii) amplification primers, (iii) amplification reagents including enzymes required for amplification, and (iv) at least one polyol capable of stabilizing the enzymes required for amplification; (b) denaturing the nucleic acids by heating the mixture at a temperature above 41&#xb0; C.; and (c) transcriptionally amplifying at least one target nucleic acid at a temperature above 41&#xb0; C. when present in the mixture.

Method for transcriptional amplification of nucleic acids combining steps of different temperatures

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Genas, France

Laurent Mesta