Firmenich Sa

Proteus S.a.

Ifp Energies Nouvelles

Proteus Gmbh

Pcas Biosolution

Laurent Fourage

Denis Wahler

Fabrice Lefevre

Antoine Margeot

Jean-Michel Masson

Daniel Dupret

Fredi Bruhlmann

Hugues Mathis

Gerard Guillamot

Juliette Martin

Nicolas Lopes-Ferreira

Fredi Brühlmann

Matthieu Chodorge

The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more particularly to beta-glucosidase variants comprising at least one modification among the amino acids located at positions 225, 238, 240 and 241, according to the numbering in SEQ ID No. 2 ofbeta-glucosidase, and also relates to the use of said variants having improved effectiveness in methods for cellulose decomposition and biofuel production.

Beta-glucosidase variants having improved activity, and uses thereof

The invention relates to an enzymatic acylation method including at least the following steps of: contacting at least one compound having at least one function selected from among the amine, alcohol, or thiol functions, at least one microorganism having an acyl transfer activity and/or an acyl transfer enzyme, and at least one acylphosphonate donor of formula (I), where: R is an alkyl, alkene, alkyne, aryl, or aralkyl radical, or is &#x2014;ORa, &#x2014;SRa, &#x2014;NRaRb, where Ra and Rb are identical or different and are H, an alkyl, alkene, alkyne, aryl or aralkyl radical, the alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; X is O or S; Y and Z, which are identical or different, are &#x2014;OR1, &#x2014;OR2, &#x2014;SR1, &#x2014;SR2, &#x2014;NR'1R&#x2033;1, &#x2014;NR&#x2032;2R&#x2033;2; R1, R2, R&#x2032;1, R&#x2032;2, R&#x2033;1 and R&#x2033;2, which are identical or different, are an alkyl, alkene, alkyne, aryl or aralkyl radical, said alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; and recovering the compound including at least one acyl function, said function being selected from among the amine, alcohol, or thiol functions. 

Enzymatic acylation method using an acylphosphonate donor

Fatty acid 13-hydroperoxide lyase proteins which have been modified with respect to a previously described guava 13-hydroperoxide lyase and the nucleic acid sequences encoding these proteins. Also, recombinant nucleic acid molecules for expressing the modified 13-hydroperoxide lyases and methods of using such lyases in the field of organic synthesis.

Modified 13-hydroperoxide lyases and uses thereof

The invention relates to methods of determining the mutational load of a gene bank obtained by random mutagenesis of a gene of interest by preparing a chart linking the mutational load of a gene bank obtained by random mutagenesis of a model gene with the fraction of mutated model genes observed in the bank; performing, in parallel, random mutagenesis of the model gene used for preparing the chart and the gene of interest to obtain the corresponding mutated gene banks; determining the mutational load of the gene bank obtained using the model gene on the basis of the chart plotted; and applying a correction factor to the mutational load of the mutated model gene bank to determine the mutational load in the gene bank of the mutated genes of interest.

Method of determining the mutational load of a gene library obtained by random mutagenesis of a particular gene and means for implementing same

The invention relates to a fragmentation process that depends on mismatches between two strands of parental polynucleotides. One embodiment, comprises a method for preparing polynucleotide fragments of DNA comprising formation of heteroduplex molecules by hybridizing polynucleotides. The invention also provides a method and process of forming fragments which can be used with any shuffling process or combination of shuffling processes.

Method of preparing polynucleotide fragments for use in shuffling, and shuffling of same

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Calvisson, France

Laurent Fourage