Biomerieux, Inc.

Biomerieux Sa

Commissariat a L'energie Atomique

Stmicroelectronics S.r.l.

Centre National De La Recherche Scientifique

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Université Claude Bernard Lyon I

Laurent Drazek

Frédéric Pinston

Bruno Colin

Hervé Rostaing

Jean-Philippe Charrier

Jean-luc Achard

Jean-Claude Raymond

Cécile Paris

Dominique Decaux

Corine Fulchiron

Frederic Ginot

Pascale Pham

Luigi Giuseppe Occhipinti

Patrick Broyer

Mathieu Dupoy

Michel Guy

Crocifisso Marco Antonio Renna

Giuseppe Emanuele Spoto

Cristian Dall'Oglio

Jean-Pierre Moy

Frédéric Mallard

Geneviève Bossy

Maëlenn Robin

Mélaz Tayakout-Fayolle

Agnès Dupont Filliard

Magaly Ponsard-Fillette

Thierry Kollaroczy

Olivier Figadere

Agnés Dupont-Fillard

Maryse Guicherd

Fabien Romanens

Isabelle Pitault

Christian Jallut

Agnès Dupont-Fillard

A method and system for measuring values of one or several characterizing parameters representative of the kinetics of an assay chemical reaction, including: providing a calibration tool having a reaction chamber; for a given set of defining features, performing a series of calibration experiments under different sets of calibration values of at least one operating parameter; providing a digital calibration model representative of the kinetics of the assay chemical reaction in the calibration tool, where the assay digital calibration model includes one or several characterizing parameters, the values of which have a dependency on the given set of defining features used for the calibration experiments; fitting, by computation, the values of the characterizing parameters for the given set of defining features, based on the series of calibration experiment results; and where the reaction chamber is a stirred-tank reactor.

Methods and systems for manufacturing a production assay reactor

A method for detecting a presence or an absence of at least one zone of inhibition, the method including a step consisting in depositing a volume of the sample in liquid form along a deposition zone extending along an axis at the surface of the agar culture medium and a step consisting in depositing a determined amount of a chemical agent at the surface of the agar culture medium, the deposit defining a potential zone of inhibition, the axis of the zone of deposition of the sample intersecting the potential zone of inhibition.

Method for detecting a presence or absence of at least one first zone of inhibition

A method detects the presence or absence of at least one first inhibition zone having a culture of biological particles on a culture medium in the presence of a chemical agent. The method of detection has a first step of seeding the culture medium with the biological particles, a second step in which the culture medium receives the chemical agent, a third step of incubation of the culture medium, and a fourth step of measurement of the first inhibition zone of the culture medium, which includes a first phase of taking an image of the first inhibition zone by ombroscopy.

Method for detecting the presence or absence of biological particles

A method and system incorporating a process for analyzing a sample to be tested to determine the presence of or to quantify an analyte in the sample. The process employs a reaction which produces a reaction medium derived from the sample that possesses fluorescence properties, in response to illumination by a light source. The process includes illuminating the sample and detecting a fluorescent signal, introducing the reaction medium, and illuminating the sample and detecting a second fluorescent signal from the sample. The process further includes processing the two signals to determine which portion of the fluorescent signal is produced solely by the reaction medium.

Method and system for detecting and measuring fluorescence signals

A locating method and system of determining a microorganism concentration of a fluid in a Petri dish is disclosed. The Petri dish includes a culture medium on which a seeding device is rotatable relative to the Petri dish. The seeding device distributes the fluid and includes at least one point of contact with the culture medium. The point of contact is associated with a contact zone.

Means, method and computer program product for determining the concentration level of microorganisms during a fluid analysis

The present invention relates to a device for preparation and/or treatment of a biological sample comprising a set of storage chambers and/or reaction chambers intended for receiving a fluid, the chambers being separated by walls so as to constitute a set of adjacent chambers. It also relates to an analysis apparatus capable of using such a device and also a method of using this device. The device comprises a base and a drawer comprising the set of adjacent chambers, the drawer being movable relative to the base, the drawer comprising a contact surface on which emerge first means for placing in fluidic communication linked to the volume of at least one chamber, the contact surface of the drawer being intended to be positioned facing a contact surface of the base comprising at least one position at which there are arranged second fluidic communication means linked to detection means. The invention has a preferred application in the field of medical diagnosis.

Device for preparing and/or treating a biological sample

A method of analyzing a sample to be tested to determine the presence of or to quantify an analyte in the sample, employing a reaction which produces a reaction medium derived from the sample and which possesses fluorescence properties, the reaction medium being located within a well, the reaction medium and the well forming an analysis assembly which possesses fluorescent properties in response to illumination by a light source producing a light signal, the light source being movable along a surface Sof the well.

The aim of the present invention is a method of sampling all or part of a sample () of biological matter (), which is crude, enriched or cultured through contact with a culture medium (), such as agar, for example in a Petri dish, using a probe () equipped with a terminal end (), said sampling method comprising the steps of cooling the terminal end () of the probe (), sticking all or part of the sample () of biological matter () to be sampled through contact, or by applying a pressure exerted by the terminal end () onto the sample () of biological matter (), and sampling of all or part of the sample () of biological matter () so as to separate the sample () of biological matter () from the culture medium (), a method of depositing into a container () or onto an analysis plate () all or part of a sample () of biological matter () stuck onto a frosted terminal end () of a probe, said depositing method including a step of separating the terminal end () of the probe () from all or part of the sample () of biological matter (), as well as a device (), a kit and an apparatus () implementing these methods.

Method of sampling and/or depositing a sample of biological matter and device implementing such method

A microfluidic device (-), comprising: a semiconductor body () having a first side () and a second side () opposite to one another, and housing, at the first side, a plurality of wells (), having a first depth; an inlet region () forming an entrance point for a fluid to be supplied to the wells; a main channel () fluidically connected to the inlet region, and having a second depth; and a plurality of secondary channels () fluidically connecting the main channel to a respective well, and having a third depth. The first depth is higher than the second depth, which in turn is higher than the third depth. According to an aspect, the microfluidic device further comprises a cover layer (), arranged above the first side of the semiconductor body, configured for sealing the wells and provided with at least a first valve hole () which extends through the cover layer and overlaps, at least partially, the secondary channels; and a flexible layer (), arranged above the cover layer and provided with at least a protrusion () extending through the first valve hole towards the semiconductor body and overlapping, at least partially, the secondary channels, the flexible layer being configured such that, when a pressure is applied on it, the protrusion contacts the semiconductor body and enters the secondary channels thus fluidically isolating the wells from one another.

Microfluidic PCR device

The present invention relates to a disposable device () for amplifying at least one target nucleic acid present in a liquid and biological sample of interest, which consists of a solid body (), at least one fluid channel () connecting an inlet (), via which all or part of the sample of interest can be drawn up and/or discharged, and an outlet (), which is itself connected to a means for the drawing up/discharging of the said sample of interest, the fluid channel () further comprising from the inlet () to the outlet (): The invention also proposes an amplification method using such a device. The said invention has a preferred application in the field of medical diagnosis.

Simplified device for nucleic acid amplification and method for using same

Method of sampling and/or depositing a sample of biological matter and device implementing such a method

The invention relates to a method for detecting clusters of biological particles () on a surface (), comprising steps that involve: a. determining (E) a topographical representation () of said surface; and b. detecting (E, E), on said topographical representation, at least one contour defining a region that is likely to correspond to a cluster of biological particles.

Method for detecting clusters of biological particles

The invention concerns a device for isolating, on a reactive surface, an analyte from a liquid sample containing the analyte.

Method and device for isolating and/or determining an analyte

The invention concerns a method for isolating an analyte from an initial sample containing same, on a reactive surface in contact with all or part of the initial sample, characterized in that it comprises the following steps which consist in: 1) forming and/or maintaining a reaction volume entity, comprising an internal liquid medium, corresponding to all or part of the initial sample, the entity including an interface with an external medium, having with the latter a surface tension; 2) applying a temperature difference between at least two thermal points located: a) proximate to and/or b) on the surface and/or c) within said entity, said thermal points being respectively different, in terms of temperature and positioning; 3) fixing the analyte on the reactive surface, positioned on the path of forces convection generated by the temperature difference.

Method and device for isolation and/or determination of an analyte

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Grenoble, France

Laurent Drazek