Corning Incorporated

Laurent A G Picard

Brian L Webb

Ye Fang

Fang Lai

Bernice I Feuer

Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

Method and device for protein delivery into cells

A cell transfection apparatus and methods for making and using the cell transfection apparatus are described. The cell transfection apparatus has a surface on which there is printed at different locations one or more foreign biomolecules (e.g., DNA, RNA, oligonucleotides, nucleotides). The foreign biomolecules can be printed at the same time. The surface is then covered with a transfection reagent which is incubated and removed from the surface before cells in a cell growth media are placed on the surface. The surface is configured such that the cells which become transfected with one or more foreign biomolecules are segregated from the cells which fail to become transfected with one or more foreign biomolecules. There are disclosed two embodiments of the cell transfection apparatus.

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Corning, NY, United States of America

Laurent A G Picard