Johns Hopkins University

Boston University

Laurene Cheung

William R Bishai

John R Murphy

Cynthia K Bullen

Shashank Gupta

Ruchi Jain Dey

Bappaditya Dey

Drew M Pardoll

Juan Fu

Pankaj Kumar

Amit Kumar

Sadiya Parveen

Vijayan Sambasivam

Sumit Siddharth

Dipali Sharma

The present invention is related to a method of treating or preventing cancer in a subject comprising administering to a subject having cancer or prone of getting cancer a first agent that depletes the subject's regulatory T cells (Tregs); followed by administering to the subject a second agent comprising a checkpoint inhibitor.

Methods of treating or preventing cancer with an agent that depletes tregs and a checkpoint inhibitor

The present invention is a DNA expression vector comprising: a toxP: a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.

Methods of producing aggregate-free monomeric diphtheria toxin fusion proteins and therapeutic uses

The present invention includes the discovery of a strain ofcomprising an expression vector encoding a di-adenylate cyclase enzyme. Theis selected from the group consisting of, or a combination thereof and the preferred strain ofis BCG. The preferred expression vector is a mycobacterial expression vector including an hsp60 promoter and a DNA sequence of diadenylate cyclase (disA), or a functional part thereof. The strains ofare used in therapeutic applications including tuberculosis and cancer.

Methods of treating cancer using bacteria expressing c-di-AMP

The present invention is a DNA expression vector comprising: a toxP; a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.

Described herein are methods of treating or preventing cancer in a subject comprising administering to the subject an effective amount of a bacteria comprising and expression vector encoding a di-adenylate cyclase enzyme.

Bacteria over-expressing c-di-AMP and therapeutic methods

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Baltimore, MD, United States of America

Laurene Cheung