Metabolic Explorer

Evonik Degussa Gmbh

Laurence Dumon-Seignovert

Philippe Soucaille

Wanda Dischert

François Voelker

Rainer Figge

Céline Raynaud

Gwénaëlle Bestel-Corre

Perrine Vasseur

Gwenaëlle Corre

Isabelle Meynial-Salles

Nadège Dumoulin

Laetitia Faure

Gwenaëlle Bestel-Corre

The present invention concerns a new mutant strain ofcomprising attenuated glycerol kinase activity. In addition, the present invention concerns a consortium ofcomprising at least said mutant strain and at least one other species ofchosen amongand. As this modified strain may be adapted for growth and for the production of 1,3-propanediol in an appropriate culture medium with high glycerol content, the invention also relates to a method for the production of 1,3-propanediolandbutyric acid, by culturing at least this mutant strain in an appropriate culture medium.

Microorganisms with improved 1,3-propanediol and butyric acid production

The present invention relates to new methylglyoxal reductase (MGR) enzymes which are useful for efficiently converting methylglyoxal into hydroxyacetone. The invention more particularly relates to a method for efficiently converting methylglyoxal into hydroxyacetone using said enzymes, to a method for producing 1,2-propanediol using a microorganism overexpressing said enzymes, and to said microorganism.

Conversion of methylglyoxal into hydroxyacetone using novel enzymes and applications thereof

The present invention relates to a recombinant microorganism capable of producing 2,4-dihydroxybutyrate, which is characterized by an increased cellular export, and preferably by a decreased cellular import, of said 2,4 DHB. The invention also relates to a method for the optimized production of 2,4-dihydroxybutyrate by culturing said microorganism in a fermentation medium and recovering 2,4-DHB from said medium.

Modified microorganism for the optimized production of 2,4-dihydroxybutyrate with enhanced 2,4-dihydroxybutyrate efflux

The present invention relates to a genetically modified microorganism for the fermentative conversion of levulinic acid into propionyl-CoA and acetyl-CoA, and to a fermentation process for performing said conversion.

Microorganism modified for the assimilation of levulinic acid

The present invention relates to a genetically modified microorganism for the production of 2,4-dihydroxybutyrate, by metabolic transformation of xylose via the 1,2,4-butanetriol intermediate. The invention also relates to a method for the production of 2,4-dihydroxybutyrate by culturing said genetically modified microorganism in a fermentation medium and recovering 2,4-DHB from said medium.

Modified microorganism for the optimized production of 2,4-dihydroxybutyrate

The present invention is related to a recombinant microorganism optimized for the fermentative production of methionine and/or its derivatives, wherein in said recombinant strain, the methionine efflux is enhanced by overexpressing the homologous logous genes of ygaZ and ygaH genes from. It is also related to a method for optimizing the fermentative production of methionine or its derivatives comprising the steps of: a. culturing a recombinant microorganism wherein in said microorganism, the methionine efflux is enhanced by overexpressing the ygaZH homologous genes of ygaZ and ygaH genes from, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and b. recovering methionine and/or its derivatives from the culture medium.

Method and microorganism for methionine production by fermentation with improved methionine efflux

The present invention is related to a recombinant() strain optimised for the fermentative production of methionine and/or its derivatives, wherein in said recombinant strain, the methionine import is attenuated and the methionine efflux is enhanced. It is also related to a method for optimising the fermentative production of methionine or its derivatives comprising the steps of: a. culturing a recombinant microorganism wherein in said microorganism, the methionine import is attenuated and the methionine efflux is enhanced, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and b. recovering methionine and/or its derivatives from the culture medium.

Microorganism for methionine production with enhanced methionine efflux

The present invention concerns a method for the production of 1,2-propanediol, comprising culturing a microorganism modified for an improved production of 1,2-propanediol in an appropriate culture medium and recovery of the 1,2-propanediol which may be further purified wherein the microorganism expresses a glycerol dehydrogenase (GlyDH) enzyme the inhibition of which activity by NAD+ and/or its substrate and/or its product is reduced. The present invention also relates to a mutant glycerol dehydrogenase (GlyDH) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein

Mutant glycerol dehydrogenase (GlyDH) for the production of a biochemical by fermentation

The present invention concerns a method for the production of a biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol comprising culturing a microorganism modified for an improved production of the biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a YqhD enzyme which catalytic efficiency toward NADPH is increased. The present invention also relates to a mutant YqhD enzyme comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein

Mutant YqhD enzyme for the production of a biochemical by fermentation

The present invention concerns a method for the production of a biochemical selected among lactic acid, acetol and 1,2-propanediol, comprising culturing a microorganism modified for an improved production of the biochemical selected among lactic acid, acetol and 1,2-propanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a methylglyoxal synthase (MGS) enzyme which activity is not inhibited by orthophosphate. The present invention concerns a mutant methylglyoxal synthase (MGS) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein

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Pont du Chateau, France

Laurence Dumon-Seignovert