Lumora Limited

Promega Corporation

Roche Molecular Systems, Inc.

Lumor Limited

Laurence Carlo Tisi

James A H Murray

Cathal Joseph McElgunn

Olga Gandelman

David James Squirrell

Christopher Robin Lowe

Peter J White

Rachel Louise Price

Melenie Jane Murphy

Guy Kiddle

James Augustus Henry Murray

Clint Pereira

Victoria Louise Church

Rachel Louise Leslie

Peter John White

Melanie Jane Murphy

Olga Gandelmann

Cathal Joseph Mcelgunn

James Augustus Murray

James A Murray

Cathal Mcelgunn

The present invention is in the field of sample preparation. In particular, it relates to methods for preparing samples prior to performing nucleic acid amplification.

Methods for preparing samples for nucleic acid amplification

The invention is in the field of nucleic acid amplification, hi particular, methods are described which utilize stem primers that improve the rapid and specific amplification of a test sample.

Stem accelerated isothermal nucleic acid amplification technology

A method for determining the presence and/or amount of a first polynucleic acid in a sample comprising subjecting the sample to nucleic acid amplification in which product is detectable by the presence of a signal generated by polynucleic acid formation from the first polynucleotide characterised in that the nucleic acid amplification reaction is conducted, in the same reaction vessel, with a predetermined amount of a second polynucleic acid which is subjected to nucleic acid amplification, the product of which is detectable by the presence of the same signal generated by polynucleic acid formation from the second polynucleotide as that generated by polynucleic acid formation from the first polynucleotide and wherein the product of the second polynucleic acid is produced with different reaction kinetics from the product of the first polynucleic acid such that the second polynucleic acid acts as an internal control for the method.

Nucleic acid amplification

An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase fromor. In the sequence of the recombinant luciferase, the amino acid residue corresponding to phenylalanine 295 inwild-type luciferase or to leucine 297 inorwild-type luciferases, is mutated compared to the corresponding amino acid which appears in the corresponding wild-type luciferase sequence. The recombinant luciferase has increased thermostability compared to the corresponding wild-type luciferase.

Mutant luciferase

A method for determining the amount of template nucleic acid present in a sample comprising the steps of: i) bringing into association with the sample all the components necessary for nucleic acid amplification, and all the components necessary for a bioluminescence assay for nucleic acid amplification and subsequently: ii) performing the nucleic acid amplification reaction; iii) monitoring the intensity of light output from the bioluminescence assay; and iv) determining the amount of template nucleic acid present in the sample.

Method for determining the amount of template nucleic acid present in a sample

A protein having luciferase activity and at least 60% similarity to luciferase fromororwherein in the sequence of the enzyme, at least one of (a) the amino acid residue corresponding to residue 214 inluciferase; (b) the amino acid residue corresponding to residue 232 inluciferase; (c) the amino acid residue corresponding to residue 295 inluciferase; (d) the amino acid residue corresponding to acid 14 ofluciferase; (e) the amino acid residue corresponding to amino acid 35 ofluciferase; (f) the amino acid residue corresponding to amino acid residue 105 ofluciferase; (g) the amino acid residue corresponding to amino acid residue 234 ofluciferase; (h) the amino acid residue corresponding to amino acid residue 420 ofluciferase; (i) the amino acid residue corresponding to amino acid residue 310 ofluciferase; is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.

[object Object]

A method for determining the amount of template nucleic acid present in a sample comprising the steps of: I) bringing into association with the sample all the components necessary for nucleic acid amplification, and all the components necessary for a bioluminescence assay for nucleic acid amplification and subsequently: ii) performing the nucleic acid amplification, iii) monitoring the intensity of light output from the bioluminescence assay; and iv) determining the amount of template nucleic acid present in the sample.

Growing community of inventors

Cambridge, United Kingdom

Laurence Carlo Tisi