New England Biolabs, Inc.

Lauren Sears Higgins

Geoffrey G Wilson

Huimin Kong

Michael A Dalton

Romualdas Vaisvila

Rebecca B Kucera

Ira Schildkraut

Ashley Luck

Rebecca Kucera

Among other things, a method for performing multiple enzyme reactions in a single tube is provided. In some embodiments, the method may comprise producing a reaction mix comprising a thermolabile UDG, an AP lyase and DNA fragments that comprise one or more uracil residues, incubating the reaction mix at a relatively low temperature to cleave fragments at the one or more uracil residues, raising the temperature of the reaction mix to a relatively high temperature to inactivate the thermolabile UDG; and deaminating the fragments, thereby converting any cytosine in the fragments of DNA to uracil.

Method for performing multiple enzyme reactions in a single tube

The present invention relates to recombinant DNA which encodes the MlyI restriction endonuclease as well as the MlyI and BstNBII methyltransferases and expression of MlyI restriction endonuclease and M.BStNBII in cells containing the recombinant DNA.

Method for cloning and expression of MlyI restriction endonuclease and MlyI methylase and BstNBII methylase in E. coli

The present invention relates to recombinant DNA which encodes the PleI restriction endonuclease as well as PleI and BstNBII methyltransferase and expression of PleI restriction endonuclease and M.BstNBII in cells containing the recombinant DNA.

Method for cloning and expression of PleI restriction endonuclease and PleI and BstNBII methylases in E. coli

The present invention relates to the recombinant DNA which encodes the SwaI restriction endonuclease, modification methylase, and the production of SwaI restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.

Method for cloning and producing the SwaI restriction endonuclease

The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI in non-thio strand displacement amplification, is disclosed also.

Cloning and producing the N.BstNBI nicking endonuclease

The present invention relates to the recombinant DNA which encodes the DraIII restriction endonuclease modification methylase, and the production of DraIII restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 vector which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 vector which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.

Method for cloning and producing the DraIII restriction endonuclease

The present invention relates to the recombinant DNA which encodes the SgrAI restriction endonuclease and the MspI modification methylase, and the production of SgrAI restriction endonuclease from the recombinant DNA.

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Essex, MA, United States of America

Lauren Sears Higgins