Novartis Finance Corporation

The United States of America As Represented by the Secretary of

The Ohio State University

Syngenta Investment Corporation

Laura S Privalle

Lyle Dean Crossland

Stephen A Goff

Patrick F Dowd

Juan J Estruch

Stephen Arthur Goff

Martha S Wright

Lawrence Mark Lagrimini

Martha Beale Hill

The present invention is drawn to a method of controlling gene expression in plants. Specifically, the method comprises obtaining a transgenic plant comprising at least two receptor expression cassettes and at least one target expression cassette. The first receptor expression cassette comprises a nucleotide sequence for a 5' regulatory region operably linked to a nucleotide sequence which encodes a first receptor polypeptide, and a 3&#x2032; termination region. The second receptor expression cassette comprises a nucleotide sequence for a 5&#x2032; regulatory region operably linked to a nucleotide sequence which encodes a second receptor polypeptide, and a 3&#x2032; termination region. The target expression cassette comprises a nucleotide sequence for a 5&#x2032; regulatory region operably linked to a nucleotide sequence which encodes a target polypeptide, and a 3&#x2032; termination region, wherein the 5&#x2032; regulatory region of the target expression cassette is activated by the first and second receptor polypeptides in the presence of one or more chemical ligands which are complementary to the ligand binding domain of the receptor polypeptides, whereby expression of the target polypeptide is accomplished. The method is useful for controlling various traits of agronomic importance, such as plant fertility.

Control of gene expression in plants by receptor mediated transactivation in the presence of a chemical ligand

The present invention is drawn to a method of controlling gene expression in plants. Specifically, the method comprises obtaining a transgenic plant comprising at least two receptor expression cassettes and at least one target expression cassette. The first receptor expression cassette comprises a nucleotide sequence for a 5' regulatory region operably linked to a nucleotide sequence which encodes a first receptor polypeptide, and a 3' termination region. The second receptor expression cassette comprises a nucleotide sequence for a 5' regulatory region operably linked to a nucleotide sequence which encodes a second receptor polypeptide, and a 3' termination region. The target expression cassette comprises a nucleotide sequence for a 5' regulatory region operably linked to a nucleotide sequence which encodes a target polypeptide, and a 3' termination region, wherein the 5' regulatory region of said target expression cassette is activated by said first and second receptor polypeptides in the presence of one or more chemical ligands which are complementary to the ligand binding domain of said receptor polypeptides, whereby expression of said target polypeptide is accomplished. The method is useful for controlling various traits of agronomic importance, such as plant fertility.

Method of controlling the fertility of a plant

The present invention relates, in general, to methods and compositions for controlling insects in monocotyledonous plants (monocots), particularly maize. More precisely, the present invention relates to (1) a method for controlling insects comprising feeding or contacting an insect with an insecticidal amount of transgenic monocotyledonous plant cells comprising a recombinant DNA sequence comprising a coding sequence encoding peroxidase and (2) a fertile transgenic monocot plant comprising a recombinant DNA sequence comprising a coding sequence encoding peroxidase.

Methods for conferring insect resistance to a monocot using a

Control of gene expression in plants by receptor mediated

Growing community of inventors

Durham, NC, United States of America

Laura S Privalle