Cargill Inc.

Sk Innovation Co., Ltd.

Teknologian Tutkimuskeskus Vtt Oy

Teknologian Tutkimuskeskus Vtt

Natureworks LLC

Valtion Teknillinen Tutkimuskeskus

Danisco Sweeteners Oy

Danisco A/s

Laura Ruohonen

Kari Koivuranta

Aristos Aristidou

Merja Penttilä

Pirkko Suominen

Vineet Rajgarhia

Marja Ilmen

Christopher K Miller

Stacey Olson

Mervi Toivari

Merja Elisa Penttila

Sang Min Lee

Peter Richard

Outi Koivistoinen

Marja Ilmén

Merja Oja

Anne Huuskonen

John Londesborough

Kirsi Bromann

Jae Hoon Jo

Jae Yeon Park

Hans Söderlund

Heikki Ojamo

Tiina Nakari-Setälä

Chris Miller

Joong Min Park

Young Bin Seo

Kevin Roberg-Perez

Woo Chan Park

Hakan Gros

Dominik Mojzita

Mira Povelainen

Merja Pentilla

Andrei Miasnikov

Anita Teleman

Simon MoonGeun Jung

Andrew Conley

Paula Jouhten

Josef Deutscher

Marja Iimen

Simon Moon Geun Jung

Claire Plazanet-Menut

Marilyn Wiebe

Tina Nakari-Setala

Merja Penttilae

Merja PenttilÄ

Tiina Nakari-SETÄLÄ

Merja Pentillä

Håkan Gros

Provided are a novel isoprene synthase derived from sweet potato and a method of preparing isoprene using the same, and more specifically, a novel isoprene synthase derived from sweet potato, a gene encoding the isoprene synthase, a host cell transformed with the gene, and a method of preparing isoprene using the same. The isoprene synthase of the present invention may have higher isoprene productivity as compared to isoprene synthases known in the related art to thereby be effectively used in isoprene biosynthesis and preparation of an isoprene polymer using the same.

Isoprene synthase and method of preparing isoprene using thereof

Provided are a recombinant yeast producing 3-hydroxypropionic acid (3-HP) and a method for producing 3-HP using the same, more particularly, a recombinant yeast producing 3-HP, comprising an exogenous AADH gene; an endogenous or exogenous ACC gene; an exogenous MCR gene; and an exogenous HPDH gene, and producing 3-HP through [Pyruvate Acetaldehyde&#x2192;Acetyl-CoA Malonyl-CoA Malonate semialdehyde 3-HP] biosynthesis pathway, and a method for producing 3-HP using the same.

Recombinant yeast producing 3-hydroxypropionic acid and method for producing 3-hydroxypropionic acid using the same

The present invention concerns a method for producing terpenes in fungi comprising the steps of (a) providing a modified terpene biosynthetic gene cluster inside a host cell, wherein one or more of the naturally occurring genes or promoters of the cluster have been replaced, truncated or removed, (b) providing a transcription factor inside the host cell, the transcription factor activating the terpene biosynthetic gene cluster; (c) cultivating said host in conditions allowing the expression of the transcription factor activating the cluster; and optionally (d) recovering the thus produced terpene product.

Method for producing terpenes

Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species, and fermentation processes using genetically modified yeast

The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phospholipid: diacylglycerol acyltransferase (PDAT) activity and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinantcell and its construction is described.

Genetically modified fungi and their use in lipid production

Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolyzed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolyzed lignocellulosic plant biomass.

Manufacture of xylonic acid

The present invention relates to a method for producing terpenes in fungi, wherein a terpene biosynthetic gene cluster having terpene biosynthetic genes and regulatory regions operably linked to said genes is activated. The invention relates also to a terpene biosynthetic gene duster and regulatory regions of such terpene biosynthetic gene cluster usable is production of terpenes, use of regulator for regulating the terpene production and use ofFGSC A4 for producing terpenes. The method of invention provides higher yields of enriched terpene product without essential amount of side-products.

Cells of the speciesand closely related yeast species are transformed with a vector to introduce an exogenous lactate dehydrogenase gene. The cells produce lactic acid efficiently and are resistant at low pH, high lactate titer conditions.

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Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications', include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species and fermentation processes using genetically modified yeast

The invention relates to the methods of manufacturing five-carbon sugars and sugar alcohols as well as other compounds derived from pentose-phosphate pathway from readily available substrates such a hexoses using metabolically engineered microbial hosts.

Manufacture of five-carbon sugars and sugar alcohols

The present invention relates to biocatalysts that are cells, optimally of the Crabtree-negative phenotype, comprising expression vectors encoding genes heterologous to the cell that enable increased production of organic products. More specifically, the invention relates to genetically modifiedcells, methods for making thecells, and their use in production of organic products, particularly lactic acid.

Growing community of inventors

Helsinki, Finland

Laura Ruohonen