E.i. Dupont De Nemours and Company

Alliance for Sustainable Energy, LLC

E.i. Du Pont De Nemours and Company Alliance for Sustainable Energy LLC

Laura McCole

Perry G Caimi

Luan Tao

Paul Veikko Viitanen

Min Zhang

Yat-Chen Chou

Mary Ann Franden

Yuying Zhang

Carol M McCutchen

Jean-Francois Tomb

Carol Mccutchen

Xylose-utilizingstrains studied were found to accumulate ribulose when grown in xylose-containing media. Engineering these strains to increase ribose-5-phosphate isomerase activity led to reduced ribulose accumulation, improved growth, improved xylose utilization, and increased ethanol production.

Xylose utilization in recombinant zymomonas

Strains ofwere engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of theglyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.

Zymomonas with improved xylose utilization

Identified are mutants of the promoter of theglyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in, and other related bacteria.

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Laura McCole