The Scripps Research Institute

International Aids Vaccine Initiative

Theraclone Sciences, Inc.

Laura Majorie Walker

Jennifer L Mitcham

Dennis R Burton

Ole Olsen

Matthew Moyle

Po-Ying Chan-Hui

Sanjay K Phogat

Wayne C Koff

Steven Frey

Stephen Kaminsky

Pascal Raymond Georges Poignard

Melissa Danielle De Jean De St Marcel Simek-Lemos

Katherine Doores

Michael Huber

The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.

Methods of treating HIV-1 infection utilizing broadly neutralizing human immunodeficiency virus type 1 (HIV-1) GP120-specific monoclonal antibodies

Broadly neutralizing human immunodeficiency virus type 1 (HIV-1) GP120-specific monoclonal antibody

Growing community of inventors

San Diego, CA, United States of America

Laura Majorie Walker