E.i. Dupont De Nemours and Company

Laura Anne Bedzyk

Rick Weizhang Ye

Pierre E Rouviere

Stephen R Fahnestock

Tanja Maria Gruber

Richard C Ebersole

Daniel P O'Keefe

Daniel P Okeefe

Raymond E Jackson

Stephen Varkey

Tao Wang

Daniel R Demarco

Daniel DeMarco

An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.

Acid-cleavable linkers exhibiting altered rates of acid hydrolysis

Methods for the direct detection of diagnostic target RNA have been developed, which obviate the need for time consuming RNA purification and isolation procedures.

Method for the direct detection of diagnostic RNA

Genes have been identified in the Bacillus genome that are responsive to various metabolic conditions and growth cycle changes. The new responsiveness of these genes allows for their use in regulated gene expression in Bacillus sp. and for the monitoring of bioreactor health.

Natural promoters for gene expression and metabolic monitoring in bacillus species

This invention relates to the isolation of nucleic acid fragments from Pseudomonas sp. strain G-179 that encode periplasmic nitrate reductase and nitric oxide reductase enzymes. The enzymes are useful in denitrification reactions and for the identification of other denitrifying bacteria. In addition, this invention also relates to the construction of chimeric genes encoding all or a substantial portion of a bacterial nitric oxide reductase or a bacterial periplasmic nitrate reductase enzymes, in sense or antisense orientation, wherein the expression of the chimeric genes results in production of altered levels of the nitric oxide reductase or periplasmic nitrate reductase in a transformed host cell.

Genes encoding denitrification enzymes

Genes encoding denitrification reactions

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Odessa, DE, United States of America

Laura Anne Bedzyk