Viacyte, Inc.

Dexcom, Inc.

Cythera, Inc.

W. L. Gore & Associates, Inc.

Laura A Martinson

Chad Green

Michael J Scott

Evert Kroon

Vincent So

Emmanuel Edward Baetge

Kevin Allen D'Amour

Donald Koenig

Erik D Olson

Giacomo Strollo

Craig McGreevy

Val Anthony Bellora

Richard Alexander Grant

Michael Scott

Leah Elliott

Craig Mcgreevy

Alan D Agulnick

Gustavo Prado

Mario Zamarripa

James H Brauker

Paul V Neale

Victoria E Carr-Brendel

Mark Allan Tapsak

Olivia Kelly

Christopher R Folk

Greg Rusch

Kevin D'amour

Lauren R Zambotti

Qiang (john) Zhang

Christopher Folk

Lauren Zambotti

Karmi Robison

Joseph Kakkassery

Qiang (John) Zhang

Timothy M Bruhn

Scott A Ritrovato

David Winer

Edward Gunzel

Embodiments herein describe tools, instruments and methods for aseptic loading, dispensing and/or delivering cells into an implantable device and aseptically and selectively sealing a device inside a sterile package as well as and storing and preparing for shipment the cell-filled device.

Instruments and methods for loading cells into implantable devices

A biocompatible membrane composite including a cell impermeable layer and a mitigation layer is provided. The cell impermeable layer is impervious to vascular ingrowth and prevents cellular contact from the host. Additionally, the mitigation layer includes solid features. In at least one embodiment, mitigation layer has therein bonded solid features. In some embodiments, the cell impermeable layer and the mitigation layer are intimately bonded or otherwise connected to each other to form a composite layer having a tight/open structure. A reinforcing component may optionally be positioned external to or within the biocompatible membrane composite to provide support to and prevent distortion. The biocompatible membrane composite may be used in or to form a device for encapsulating biological entities, including, but not limited to, pancreatic lineage type cells such as pancreatic progenitors.

Biocompatible membrane composite

Disclosed herein are implantable 3-dimensional large capacity device assemblies, specifically, large capacity device assemblies for encapsulating pancreatic progenitor cells for treatment of diabetes.

3-dimensional large capacity cell encapsulation device assembly

Disclosed are cell encapsulation devices and methods for transplanting cells, such as pancreatic endoderm cells, into a host. In some examples, a cell encapsulation can comprise a lumen configured to receive cells therein, a cell-excluding membrane, where the lumen is internal to the cell-excluding membrane, and a non-woven fabric layer external to the cell-excluding membrane, where the non-woven fabric layer and the cell-excluding membrane comprise perforations. The device can further comprise a woven mesh external to the non-woven fabric layer, where the non-woven fabric layer provides protection to the cell-excluding membrane from direct contact with the woven mesh.

PDX1 pancreatic endoderm cells in cell delivery devices and methods thereof

Embodiments herein describe tools and instruments for holding, transferring, delivering, deploying an implantable device and methods and means of aseptically storing and shipping the implantable device including but not limited to a device case for protecting, housing and filling the device, a surgical sizer for preparing the implantable site, a deployer for transferring the implantable device from the device case and delivering or deploying the implantable device at the prepared implantable site.

Tools and instruments for use with implantable encapsulation devices

Disclosed herein are methods of producing pancreatic hormone-expressing cells by first differentiating pluripotent cells in cell culture so as to produce endodermal cells, the endodermal cells being competent to further differentiate into hormone-expressing cells capable of secreting at least one pancreatic hormone in response to a physiological signal, and then, transplanting the cultured endodermal cells into an organism, such as an organism in need of an endocrine cell therapy.

Methods of producing pancreatic hormones

The present invention relates to a cryopreserved in vitro cell culture comprising human pancreatic progenitor cells that co-express pancreatic-duodenal homeobox factor-1 (PDX1) and NK6 homeobox 1 (NKX6.1) and are chromogranin negative. The present invention also relates to a method for cryopreserving an in vitro population of human pancreatic progenitor cells that co-express PDX1 and NKX6.1 and are chromogranin negative.

Cryopreserved in vitro cell culture of human pancreatic progenitor cells

Disclosed are devices and methods for transplanting cells, such as pancreatic endoderm cells, into a host. The devices include a non-woven fabric external to a cell-excluding membrane, and the non-woven fabric and/or cell-excluding membrane can be perforated. Treatment of the host with immunosuppressive reagents, required to inhibit allograft rejection due to perforations in the cell delivery device, does not compromise maturation or function of transplanted pancreatic endoderm cells.

Disclosed herein are methods for cryopreserving, hibernation and room temperature storage of PEC aggregates, implantable semipermeable devices and the VC combination product.

Cryopreservation of encapsulated pancreatic endoderm cells

Methods of lowering blood glucose levels in a mammal

Growing community of inventors

La Mesa, CA, United States of America

Laura A Martinson