Reanal Finomvegyszergyar

Villamosenergiapari Kutato Intezet

Tatabanyai Hoeromu Vallalat

Szeszipari Kutatointezet

Laszlo Boross

Bela Szajani

Ivan Daroczi

Jozsef Szejtli

Pal Resch

Miklos Kalman

Karoly Remenyi

Gabor Seres

Ferenc Horvath

Laszlo Voros

Imre Suto

Aranka Kiss Nee Deer

Erzsebet Dala Nee Rezneki

Zsuzsanna Antal Nee Magyar

Sandor Kovacs

Gabriella Klamar

Arpad Tetzli

Tibor Gerlai

Kamilla Kovacs

Elemer Laszlo

Laszlo Ludwig

Jeno Kurtossy

Agoston Hoschke

Bela Szanjani

Ivan Darozi

Katalin Ivony Nee Kaldive

Eva Beller

Hybrid fluidized bed and pulverized coal combustion system and a process

Glucoamylase is immobilized with a carbodiimide on a polymer having amino or carboxy functional groups. When the polymer contains amino groups, the glucoamylase is treated with the carbodiimide and then mixed with the polymer or the carbodiimide is added to a mixture of the glucoamylase and the polymer. When the polymer contains carboxy groups, the polymer is treated with the carbodimide and then mixed with the glucomylase. The polymer is produced from acrylic acid, metharylic acid, acrylamide, methacrylamide, N-amino-ethyl-acrylamide and/or N-amino ethyl methacrylamide and a cross-linking agent of the acrylic or allylic type.

Process for immobilization of glucoamylase

The invention relates to a process for the immobilization of compounds comprising nucleophilic groups. According to the process of the invention a polymer comprising an amido group is swollen in a buffer. For this purpose any buffer is suitable which contains no amino, sulfhydryl and/or hydroxy groups in the molecule. To the swollen polymer a solution of a quinone - preferably p-benzoquinone - in a water miscible organic solvent is added and the mixture is activated at 273.degree.-343.degree. K. for 0.5-48 hours - preferably for 24 hours. The unreacted quinone is washed out with an aqueous solution of a water miscible organic solvent and thereafter water and/or a buffer. To the purified gel at a pH value between 3 and 11 - preferably between 6 and 8 - a buffered solution of a compound comprising a nucleophilic group is added, the suspension is incubated at 260.degree.-313.degree. K. for 24 hours, the gel is separated and the non-bound compound comprising a nucleophilic group is removed by washing.

Process for the immobilization of compounds comprising nucleophilic

Carboxypeptidase B enzyme is isolated by a process wherein homogenized and autolyzed pancreas is subjected to a thermal treatment, and carboxypeptidase B is separated by fractionation with ammonium sulfate. This process produces carboxypeptidase B having satisfactory specific activity while eliminating the use of pancreas cell fluid or an acetonous powder from pancreas as a starting material. The isolated carboxypeptidase B may be immobilized on a polymer gel containing carboxy groups activated with a carbodiimide.

Isolation of carboxypeptidase B enzyme

The invention relates to a process for the preparation of a cyclodextrine glycosyltransferase enzyme. The immobilization can be carried out by two methods. According to the first method a cyclodextrine glycosyltransferase enzyme is activated with a solution of a carbodiimide compound and the thus treated enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polysaccharide derivative comprising at least 0.1 m-equiv./g of free amino group. According to the second method the enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polymer produced from an acrylic acid and/or methacrylic acid or acrylic amide and/or methacrylic amide monomer by means of a cross-linking agent of the acrylic or allylic type which polymer comprises at least 0.1 m-equiv./g of functional carboxy group, activating the polymer with a solution of a carbodiimide compound and washing and if desired drying the product thus obtained. The advantage of the process of the present invention is that the thus immobilized enzyme--contrary to the dissolved enzyme--is suitable for longlasting application and can be readily used on industrial scale continuous production.

Process for the immobilization of cyclodextrine glycosyltransferase

Immobilized cholinesterase enzyme preparations and a process for the

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Budapest, Hungary

Laszlo Boross