Other

Pharmacia Biotech Ab

Akesson, Elisabet

Laurell, Thomas

Wallman, Lars

Sundström, Erik

Lars Wallman

Thomas Laurell

Johan Nilsson

Johan Drott

Staffan Nilsson

Elisabet Åkesson

Erik Sundström

The invention relates to a biological microglia comprising at least two pores having a size adopted to allow cells, cell aggregates, tissue or other biological material to pass through said pores, and one or several slicing beams separating said pores from each other, wherein biological material is split/sliced/cleaved into at least two parts when passing said microgrid, a slicing device, an apparatus comprising said slicing device as well as the use of said microgrid, slicing device and apparatus.

Slicing device

The invention relates to a method for etching an opening, and more precisely, to etching in a silicon plate for creating a nozzle opening. According to the invention, one side of the silicon plate ( ) is protected by a protective layer ( ), and a recess ( ) is made in the protective layer. Etching is made anisotropically through the recess so as to create a cavity ( ) in the shape of a truncated pyramid of a predetermined depth in the silicon plate. The cavity is doped so as to create a doped layer ( ) at the predetermined depth. The etching is then continued until the bottom surface of the cavity has passed the doped layer. Subsequently, etching is performed from the other side, while a voltage is applied to the doped layer, so as to free the nozzle opening at the other side. The invention enables an accurate control of the surface area of the nozzle opening. Through this, the amount of discharged fluid and the directional precision can be controlled very accurately.

Method of etching an opening

A sampling cell of flow-through type and use of such a sampling cell. The sampling cell is preferably manufactured by etching of silicon wafers. It is especially useful for continuous picovolume sampling in an analytical flow. The pressure pulse generator (,) generates pulses directly into a flow channel (,). The flow channel (,) is preferably formed by a first basin (,) in a first structure (,) and a second basin (,) in a second structure (,). In a first embodiment the pressure pulse generator (,) comprises at least one piezo-ceramic disc and/or devices acting by way of magnetostrictive, electrostatical or electromechanical forces and/or devices acting by way of thermal expansion. Method of directing samples from a flow-through sampling cell by establishing a difference in electrical potential between the liquid in the flow-through sampling cell and the object to which the samples are to be directed. Use of a flow-through sampling cell for coating of surfaces, especially for achieving biospecific surfaces, for extracting samples from a continuous liquid flow, for extracting a precise sample amount by collecting a defined number of samples or for injecting samples for electrophoresis, especially capillary electrophoresis, and for electrochromatography.

Flow-through sampling cell and use thereof

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Vollsjö, Sweden

Lars Wallman