Novo Nordisk A/s

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Fisher Bioimage Aps

Bioimage A/s

Lars Kongsbak Poulsen

Ole Thastrup

Sara Petersen Bj&oslash;rn

S&oslash;ren Tullin

Sara Petersen Bjorn

Soren Tullin

Sara Petersen Bjørn

Sara Petersen Bjo Slashedrn

Søren Tullin

So Slashedren Tullin

The present invention relates to novel variants of the fluorescent protein GFP having improved fluorescence properties.

Fluorescent proteins

The present invention relates to a method of detecting biologically active substances affecting intracellular processes, an isolated DNA, a vector and a cell for use in the method. More specifically, the present invention provides a method for detecting a biologically active substance that affects intracellular processes mediated through a protein kinase or a second messenger, which comprises incubating a cell with a test substance and measuring any change caused by the test substance in the flourescence of (i) a wild-type or modified green flourescent protein (GFP) having a protein kinase recognition site, (ii) a modified GFP containing a second messenger binding domain, or (iii) a hybrid polypeptide having a wild-type or modified GFP and an attached protein kinase recognition site or a second messenger binding domain.

Method of detecting biologically active substances

The present invention provides a method for detecting a biologically active substance that affects intracellular processes mediated through a protein kinase or second messenger, which comprises incubating a cell with a test substance and measuring any change caused by the test substance in the fluorescence of (i) green fluorescent protein (GFP) having a protein kinase recognition site or (ii) GFP that has been modified to contain a second messenger binding domain.

Method of detecting biologically active substances by using green

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Holte, Denmark

Lars Kongsbak Poulsen