Vipergen Aps

Vipergen Pharmaceuticals Aps

Lars Kolster Petersen

Nils Jakob Vest Hansen

Peter Blakskjaer

Allan Beck Christensen

Margit Haahr Hansen

Tara Renee Heitner

Frank Abildgaard Slok

Leif Kongskov Larsen

Judith Rasmussen-Dietvorst

Tara Heitner Hansen

Johan Holmkvist

Nils Jokob Vest Hansen

Ole Kristensen

Jacob Andersen

Nils Jakob Hansen

Frank Abildgaard Sloek

Provided is a method for screening an in vitro display library for binding within a cell of a small-molecule chemical compound binding entity of the library to a protein or RNA target of interest in order to identify at least one individual chemical compound binding entity of the library that is capable of binding within the cell to the protein or RNA target of interest.

Method for screening of an in vitro display library within a cell

A method for making an enriched library comprising specific nucleic acid sequence information allowing to identifying at least one binding entity that binds to at least one target wherein the specific binding entity has been present in an in vitro display library.

Method for making an enriched library

Disclosed is a composition comprising the nucleic acid and a chemical compound, said composition forming a star structure defining 3 or more stems extending from a reaction center. The stems are formed by a nucleic acid duplex and the chemical compound has been formed in the reaction center as the reaction product of 3 or more chemical groups. The advantage of the composition is that a close proximity is provided between the chemical groups in the reaction center, thereby promoting a reaction. The invention also relates to a method for preparation of the composition. The advantage of the method is that it does not require the pre-synthesis of a large number of templates and that it is not dependent upon codon/anti-codon recognition for an encoded molecule to be formed.

Structural nucleic acid guided chemical synthesis

Disclosed is a composition comprising a nucleic acid and a chemical compound, said composition forming a star structure defining 3 or more stems extending from a reaction center. The stems are formed by a nucleic acid duplex and the chemical compound has been formed in the reaction center as the reaction product of 3 or more chemical groups. The advantage of the composition is that a close proximity is provided between the chemical groups in the reaction center, thereby promoting a reaction. The invention also relates to a method for preparation of the composition. The advantage of the method is that it does not require the pre-synthesis of a large number of templates and that it is not dependent upon codon/anti-codon recognition for an encoded molecule to be formed.

The present invention provides compositions, compounds and methods for in vitro DNA display technology, allowing display of a variety of molecules, in particular molecules obtained by water incompatible mechanisms. Advantages of such methods are that combinatorial libraries can be constructed comprising molecules which are obtained through water incompatible reactions.

Method for preparing compounds by nucleic acid directed synthesis

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Skaevinge, Denmark

Lars Kolster Petersen