Qiagen North American Holdings, Inc

Eppendorf Ag

Qiagen Gmbh

Lars-Erik Peters

Michael J Domanico

José Remacle

Nan Fang

Konrad Beyreuther

Anna Ettinger

Ryan Smith Westberry

Jessica Jaclyn Greenlee

Sven Buelow

The invention provides one-component and two-component DNA polymerases, as well as kits comprising the same, and methods of using the same for nucleic amplification and nucleic acid sequencing.

One component and two component DNA Pol III replicases and uses thereof

The present invention relates to a thermophilic polymerase, wherein the DNA polymerase has an in-vitro primer extension rate that is &#x3e;35 bases/second and faster relative to the primer extension rate of a DNA polymerase comprising amino acid sequences SEQ ID NO: 2 or 4, when measured under identical conditions in a DNA replication assay using primed single strand M13mp18 DNA and an incubation temperature of 60&#xb0; C. The invention also relates to a vector comprising the polymerase, a host cell comprising the vector. The invention relates to a nucleic acid replication kit comprising the polymerase according to the invention.

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Methods and compositions are provided for enhanced specificity and sensitivity of amplification reaction mixtures. Compositions in accordance with the present invention provide for reduced formation of primer-aggregates during amplification reactions. Reaction mixes include dNTPs, where a portion of the dNTPs has been replaced with an unconventional nucleotide, e.g., dUTP. Unconventional nucleotide concentrations are typically between 10% to 50% of the concentration of one of the standard dNTP. In some compositions the unconventional nucleotide is dUTP which replaces from about 10% to about 50% of the dTTP in the dNTP mix.

dUTP-based compositions for reducing primer-aggregate formations during nucleic acid amplification

The present invention relates to a detection and quantification method of different sets of target molecules (), being possibly present simultaneously in a biological sample by the use of a microarray present upon a solid support surface () and comprising different sets (A, B) of capture molecules (), present in different locations () of the solid support surface ().

Customized micro-array construction and its use for target molecule detection

Methods and compositions are provided for amplifying RNA from an RNA template. Methods and compositions are further provided for detecting an RNA in an RNA containing sample. Compositions used for the amplification of RNA from RNA include a RNA-dependent RNA-polymerase, a RNA helicase, and an energy source. Illustrative RNA-dependent RNA-polymerase enzymes are derived from the tomato or tobacco plant, while illustrative RNA helicase enzymes include the eIF4A and eIF4B proteins.

Methods and compositions for RNA amplification and detection using an RNA-dependent RNA-polymerase

Methods for improving sensitivity and specificity of polynucleotide synthesis are disclosed. The method includes reversibly blocking thermophilic polymerase activity with non-nucleic acid polyanions in a temperature dependent manner. The methods control target specific primer extension throughout all stages of a DNA or RNA amplification reaction. Corresponding compositions and kits are disclosed.

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Lafayette, CO, United States of America

Lars-Erik Peters