Ge Healthcare Bio-sciences Ab

Pricer Ab

Amersham Biosciences Ab

Ge Healthcare Bioprocess R&d Ab

Amersham Pharmacia Biotech Ab

Other

Kai R$d Center Co., Ltd.

Biotage Ab

Cytiva Bioprocess R&d Ab

Lars C Andersson

Jean-Luc Maloisel

Bjorn Nilsson

Ronnie Palmgren

Mats Olsson

Mattias Algotsson

Hanno Ehring

Andreas Axen

Andreas Axén

Bengt Asberg

Eggert Brekkan

Lee Hedemyr

Torvald Andersson

Tesfai Gebru

Ola Lind

Patric Fricking

Bjorn Noren

Pär Rosander

Stefan D'Argy

Joachim Tollstoy

Lee Williams

John Clachan

Joakim Svensson

Anna Arefalk

Jan Forssjo

Tania Ahmad

Mikael Yttreus

Björn Nilsson

Esfir Lofman

Ulf Tedebark

Björn Norén

The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.

Chelator and use thereof

The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity. The dimeric pentadentate chelator according to the invention has the following general formula:  

The present invention relates to laboratory instruments, in particular to instruments for automated preparation of liquid samples like Solid Phase Extraction (SPE), Supported Liquid Extraction (SLE) or liquid-liquid extraction (LLE). The instrument reduces the risk of cross-contamination between sample collection containers by lifting the sample collection containers to the level of the outlet of the sample processing containers, or slightly above. The instrument further comprises a movable table positioning the liquid collection containers directly beneath said sample processing containers and a waste collection container directly beneath the sample processing containers, wherein said waste collection container is vertically movable from a position below the movable table to a position above the movable table.

Sample preparation workstation

A flow-through conductivity sensor assembly comprises a housing () having a flow passage with an inlet end and an outlet end. The cross section of at least a section of the flow passage extension between the inlet and outlet ends is divided into a conductivity measuring channel () and a parallel by-pass channel () of larger cross-sectional area than the measuring channel ().

Conductivity sensor assembly

The present invention is relates to method for production of a biomolecule adsorbent with a pentadentate ligand with very strong chelating properties, and to methods for use thereof for, for example, protein purification, detection or binding. The biomolecule adsorbent comprises the formula: wherein Q is a carrier, S is a spacer, L is an amide linkage, X is COOH, and n=2 or 3.

Method for preparation of a biomolecule adsorbent

An electronic label wirelessly connected to an information updating system having a server. The electronic label includes a wireless communication interface to receive information from the server to be displayed on a display, a means for label processing connected to the communication interface, a means for updating the display connected to the means for label processing, and at least one dot matrix display to display a dot matrix image, where the dot matrix display is connected to the means for updating the display. The communication interface receives the information to be displayed from the information updating system. The label includes a layout script such that the means for label processing generates the dot matrix image from the received information using the layout script.

System and method for updating electronic label displays

An electronic label wirelessly connected to a product information updating system. The electronic label includes a wireless communication interface to receive information from a server to be displayed on a display, a means for processing connected to the communication interface, a means for updating the display connected to the means for processing, and at least one dot matrix display to display a dot matrix image, where the dot matrix display is connected to the means for updating the display. The communication interface receives the information to be displayed from the product information updating system. At least a part of the dot matrix display displays a first barcode that can be scanned by a barcode scanner, where the first barcode identifies the electronic label or a product linked to the electronic label.

A column packing system comprises a control unit provided with software for calculating the breakpoint where a movable adapter comes into contact with a settled bed of bed media. The calculated breakpoint is used by the control unit to determine how much further the movable adapter has to move into the column in order to achieve a desired amount of bed compression.

Automated packing system and method for chromatography columns

A method for determining the concentration of a chromatographic medium in a liquid slurry comprises the steps of: providing a column () having a top end () and a bottom end () and adapted to retain a chromatographic medium therein; introducing a defined volume of the slurry () into the column (); providing a liquid flow through the column () from the top end to the bottom end of the column to settle the chromatographic medium in the column; measuring the bed height of the consolidated chromatographic medium or a value related thereto; and determining from the measured bed height or value related thereto the concentration, or a value related to the concentration of the chromatographic medium in the slurry. A device for performing the method as well as the use of the method in packing of especially large scale columns are also disclosed.

Determination of slurry concentration

An electronic label, which is wirelessly connected to a product information updating system, said label comprising at least one display () adapted to display product information. According to the invention the display () has a transparent or semitransparent backside and is adapted to be lit up from behind.

An electronic label showing information about a product related to this electronic label on a display (), said electronic label being updated from a central control unit. According to the invention the electronic label comprises a replaceable front shell () and a stationary communication part () to which the front shell should be attached.

Electronic label

The present invention relates to the preparation of a metal chelating separation medium, which comprises providing a compound defined by formula: R&#x2014;O&#x2014;CO&#x2014;(CH)&#x2014;CHNH&#x2014;CO&#x2014;OH wherein R is a selectively cleavable group; and n is an integer of 1&#x2013;4; reacting it with ethyl alcohol; derivatisation; cleavage of R; immobilisation of the resulting compound to a base matrix; and deprotection of the carboxylic acid. In one embodiment, the compound provided in the first step is ethyl ester of aspartic acid or of glutamic acid, and the derivatisation agent is bromo-acetic acid ethyl acetate.

Preparation of a metal chelating separation medium

The present invention relates to a method of generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of In the most preferred embodiment of the method, steps (c) and (d) are performed simultaneously as one single step.

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Uppsala, Sweden

Lars C Andersson