Danmarks Tekniske Universitet

Lars Blank

Harald Haeger

Thorsten Eggert

Peter Ruhdal Jensen

Peter Welters

Katrin Grammann

Bruno Buehler

Andrea Weckbecker

Katrin Schullehner

Bernd Lalla

Jensen B Koebmann

Andreas Mueller

Andreas Karau

Andreas Schmid

Volker Sieber

Thomas Haas

Guido Jach

A method of enhancing biomass yield of a lactic acid bacterial species cell culture, comprising cultivating the cells in a process comprising the steps of providing conditions that results in a reduced glycolytic flux and providing conditions that enable the cells to have, under aerobic conditions, a respiratory metabolism. The increased yield of biomass may be the result of an increased yield of ATP which can be obtained by activating the native ATP synthase activity of the H -ATPase complex by lowering the ATP/ADP ratio, e.g. by carbon source limitation, and/or by increasing the proton gradient (membrane potential) of the cells, e.g. by enhancing or establishing an electron transport chain which can be achieved by enhancing expression of dehydrogenases or electron transport chain components, by adding to the medium a quinone or porphyrin compound or by enhancing the expression of the H -ATPase activity.

Method of improving biomass yield of lactic acid bacterial cultures

Growing community of inventors

Kamen, Germany

Lars Blank