Modpro Ab

Pharmasurgics in Sweden Ab

Other

Lars Baltzer

Inger Mattsby-Baltzer

Lars A Hanson

Gunnar T Dolphin

Anders Virtanen

Ingemar Lundström

Bo Liedberg

Adam Slosarczyk

Per Ahlberg

Jonas Bergquist

Gunnar Dolphin

A molecular tool for use in a method of providing a molecule that is capable of binding a target molecule based on a set of polypeptides. A polypeptide having a sequence selected from SEQ ID NOs 1-32. The polypeptide may be used in a method of screening for a ligand-polypeptide conjugate capable of binding a target molecule for the ligand. A ligand-polypeptide conjugate, useful e.g. in therapy.

Compounds and methods

Isolated polypeptides comprising one or more of SEQ ID NOs 3-18, which show a specific binding for C-reactive protein (CRP). A library of isolated polypeptides comprising the polypeptides of SEQ ID NOs 3-18 is disclosed, as are compositions comprising such polypeptides.

Binder for C-reactive protein

A polypeptide dimer is provided wherein both protomers have a sequence according to SEQ ID NO: 1 and at least one phosphocholine derivative is attached to the polypeptide. The polypeptide shows a specific binding for C-reactive protein (CRP). The utilization of the polypeptide in assays for determining the concentration of CRP is described. The purification of CRP, and compositions comprising the CRP also are provided.

The invention relates to new peptides formed of at least seven subsequent amino acids of the amino acids in position 12-40, counted from the N-terminal end, in the sequence constituting human lactoferrin, and preferably modifications thereof. The invention also relates to medicinal products comprising such peptides, especially intended for treatment and prevention of infections, inflammations and tumours. Furthermore, the invention relates to food stuff, e.g. infant formula food, comprising the above mentioned peptides.

Peptides based on the sequence of human lactoferrin and their use

Novel peptide having a sequence according to SEQ. ID. No. 1, SEQ. ID. No. 2 and/or SEQ. ID. No. 3 are disclosed and also polypeptide scaffold consisting of a four helix bundle formed of two dimerized helix-loop-helix motifs, said helix-loop-helix motifs having sequences according to SEQ. ID. No. 1, SEQ. ID. No. 2 and/or SEQ. ID. No. 3 which may comprise a fluorescent probe at the side chain of Lys15 and a ligand with affinity for a target molecule at the side chain of Lys8 or Lys34. Also disclosed are polypeptide scaffolds for use in biosensing applications with or without the polypeptide scaffold anchored to a solid surface.

Polypeptide scaffolds and use thereof

Disclosed are novel polypeptides of a four helix bundle formed of two dimerized helix-loop-helix motifs, wherein either both have a sequence according to SEQ. ID No. 1, SEQ. ID No. 2, SEQ. ID No. 3, SEQ. ID No. 4, SEQ. ID No. 5, or SEQ. ID No. 7, or one has a sequence according to SEQ. ID No. 6, and the other one has a sequence according to SEQ. ID No. 1, SEQ. ID No. 2, SEQ. ID No. 3, SEQ. ID No. 4, SEQ. ID No. 5 or SEQ. ID No. 7. Also disclosed is a method for site-selective acylation of a folded polypeptide or protein based on the use of a four helix bundle formed of two dimerized helix-loop-helix motifs folded in an antiparallel mode, said helix-loop-helix motifs comprising amino acid residues in a heptad repeat pattern (a b c d e f g), wherein all but 1-3 of said amino acid residues in positions a and d are non-polar and wherein said 1-3 amino acid residues in positions a and d that are non-polar, are selected from the group consisting of lysine, ornithine, diaminobutyric acid and homolysine, wherein said amino acid residues in positions a and d form a hydrophobic core, wherein the polypeptide and/or protein to be acylated is placed in an aqueous solution and an acylation agent is added.

Site selective acylation

A catalytically active peptide comprising an imidazole function in position i flanked by at least one functional group to be amidated in position i+3+4k, where k is an integer equal to or higher than &#x2212;1 or in position i&#x2212;4&#x2212;4n, wherein n is an integer equal to or higher than 0, characterized in that it also comprises at least one activating group in position i+4+4n or i&#x2212;3&#x2212;4n, respectively, wherein n is as above.

Catalytically active peptides

A method for site-selective acyl transfer in amidation of folded polypeptides and proteins based on the use of a chemical structure element comprising an imidazole function, such as histidine, in position i flanked by at least one functional group to be amidated, such as lysinc, in position i+3+4k. where K is an integer equal to or higher than &#x2212;1, or in position i&#x2212;4&#x2212;4n, wherein n is an integer equal to or higher than 0, and at least one activating group, such as serine, in position i+4+4n or i&#x2212;3&#x2212;4n, respectively.

Site-selective acyl transfer

The invention relates to a method of performing a chemical reaction between a reagent and a substrate, involving an acyl transfer mechanism, in the presence of an imidazole-based catalyst capable of forming a transition complex with the substrate. The catalytic imidazole function is provided by a chemical structure element comprising an optionally substituted imidazolyl group flanked on one or both sides by a group or groups capable of stabilizing the transition complex by molecular interaction with the acyl group. The invention also relates to such a designed chemical structure element, a method of producing it by recombinant DNA techniques and a vector therefor.

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Göteborg, Sweden

Lars Baltzer