United States of America, As Represented by the Secretary of Agriculture

The United States of America As Represented by the Secretary of

University of California

The Texas A&m University System

Other

The United States of America As Represented by the United States

Larry H Stanker

John R DeLoach

Robert M Hnasko

Martin Vanderlaan

Luisa W Cheng

Bruce E Watkins

Jeffery A McGarvey

David J Nisbet

Donald E Corrier

Michael H Kogut

Miles C Scotcher

Billy M Hargis

Robin C Anderson

Xiaohua He

Ross C Beier

Rita B Moyes

Carol Kamps Holtzapple

James A Byrd, Ii

Stanley B Prusiner

Mendel Friedman

John S Vogel

Ronald H Jensen

Kenneth W Turteltaub

James S Felton

Jay C Davis

Colin R Young

William L Bigbee

Barton L Gledhill

Peter Petrovic

Siegbert Gorbach

Craig B Skinner

Cynthia L Sheffield

Kenneth J Genovese

Carol Kamps-Holtzapple

Beate G Rose

Elbert W Branscomb

Robert J Grabske

Nina R Bailey

Lisa A Gorski

Candace R Bever

Carolyn L Bigbee

Alice V Lin

Jeanette M Van Emon

Marcel H Elissalde, Jr

Loyd D Rowe, Jr

Amatoxins (AMAs) are lethal toxins found in a variety of mushroom species. Detection methods are needed to determine the occurrence of AMAs in mushroom species, often suspected in mushroom poisonings. Provided herein are novel, sensitive monoclonal antibodies (mAbs) detection and purification techniques utilizing the mAbs that show selectivity for &#x3b1;-amanitin (&#x3b1;-AMA), &#x3b2;-amanitin (&#x3b2;-AMA) and &#x3b3;-amanitin (&#x3b3;-AMA).

High affinity monoclonal antibodies for detecting amanitins

The present invention relates to the use of phyllosphere-associated lactic acid bacteria that demonstrate inhibitory effects on the growth and maintenance of human pathogens, such as, on the surface of food products, particularly fresh fruits and vegetables. Particular strains ofandare described and detailed for their atypical pathogen-inhibiting phenotypes.

Use of phyllosphere associated lactic acid bacteria as biocontrol agents to reduce bacterial growth on fresh produce

Herein is described a novel hybridoma cell line (RMH359) and its use as a cellular bioassay to determine the efficacy of cell conditioned media (CCM) as a supplement used to support cell survival and promote growth in culture. This bioassay is the first to provide a measure of CCM bioactivity in support of hybridoma cells used in the generation and production of monoclonal antibodies.

Bioassay for cell conditioned media

High affinity monoclonal antibodies against Stx2f and hybridomas that produce such antibodies are described. The antibodies may be used in a kit for detecting Stx2f and variants thereof in a sample as well as neutralization of Shiga toxin in vivo.

High affinity monoclonal antibodies for detection of shiga toxin 2

Peptide sequences that specifically bind infectious prion protein for the generation of antibodies and therapeutic agents are disclosed herein.

High-affinity monoclonal anti-prion antibodies

High affinity monoclonal antibodies against Stx2 and hybridomas that produce such antibodies are described. The antibodies may be used in a kit for detecting Stx2 and variants thereof in a sample as well as neutralization of Shiga toxin in vivo.

High affinity monoclonal antibodies for detection of Shiga toxin 2 (STX2)

High affinity antibodies for binding epitopes of BoNT/B and hybridomas that produce such antibodies are described. The antibodies may be used in a kit for detecting BoNT/B in a sample.

High-affinity monoclonal antibodies for botulinum toxin type B

High affinity antibodies for binding epitopes of BoNT/Aand hybridomas that produce such antibodies are described. The antibodies may be used in a kit for detecting BoNT/A in a sample.

High-affinity monoclonal antibodies for botulinum toxin type A

The invention provides a method and compositions for controlling food borne enteric bacterial pathogens in animals. Populations of enteropathogenic bacteria may be substantially reduced or eliminated by treatment of animals with an effective amount of the compound X (ClO ) , wherein X is a cationic moiety and m and n are independently selected from integers necessary to provide a net valency of 0. The compounds may be administered orally, providing a reduction in the populations of the enteropathogenic bacteria in the alimentary tract of the animal, or they may be applied externally onto the animal to reduce the populations of any such bacteria which may be present as contaminants on the surface of the animal. The method and compositions are particularly useful for the control of Salmonella species, enteropathogenic , and Clostridia species.

Use of chlorate ion or preparations thereof for reduction of food borne pathogens

The invention provides a method and compositions for controlling food borne enteric bacterial pathogens in poultry populations. The incidence of the colonization of poultry by enteropathogenic bacteria, and/or the populations of enteropathogenic bacteria within colonized poultry, may by substantially reduced by providing particles of an expanded matrix material to the locus or vicinity of the animals, particularly during the period of feed removal prior to slaughter. The method and compositions are particularly useful for the control of Salmonella species, enteropathogenic and Campylobacter species.

System for the control of enteropathogenic bacteria in the crops of poultry

Hybridoma cell lines have been generated which produce and secrete monoclonal antibodies which selectively bind to 4,4&prime;-dinitrocarbanilide (DNC), the active agent of nicarbazin. These hybridomas may be obtained by using as an immunization agent or immunogen, p-nitroaniline which has been conjugated to an immunogenic carrier. DNC in biological samples may be detected and quantified by contacting the sample with the antibodies to form a DNC/antibody immunocomplex when DNC is present, which immunocomplex may then be detected. The monoclonal antibodies also may be incorporated into kits for the detection and quantification of DNC and/or nicarbazin.

Monoclonal antibodies to 4,4&prime;-dinitrocarbanilide and a method for analyzing for the drug nicarbazin

The invention provides a method and compositions for controlling food borne enteric bacterial pathogens in poultry populations. The incidence of the colonization of poultry by enteropathogenic bacteria, and/or the populations of enteropathogenic bacteria within colonized poultry, may by substantially reduced by providing particles of an expanded matrix material to the locus or vicinity of the animals, particularly during the period of feed removal prior to slaughter. The method and compositions are particularly useful for the control of Salmonella species, enteropathogenic,and Campylobacter species.

A method and composition for treating swine to increase their resistance to pathogenic microorganisms are disclosed. Microbial infections may be prevented or reduced in swine populations by administration of immune lymphokines which have been produced by the splenic T cells of immunized swine. The process and compositions are particularly useful for the control of Salmonella in swine.

Immune lymphokine-mediated control of Salmonellosis in swine

Growing community of inventors

Livermore, CA, United States of America

Larry H Stanker