Monsanto Technology LLC

Dekalb Genetics Corporation

Calgene Corporation

Larry A Gilbertson

Shihshieh Huang

Xudong Ye

James Kevin Roberts

Michael W Petersen

Edwards M Allen

Thomas M Malvar

David K Kovalic

Susan Jane Johnson

James Arthur Baum

Wei Wu

Maolong Lu

Elysia K Krieger

Bei Zhang

Tichafa R I Munyikwa

David A Walters

Paul S Chomet

Sergey Ivashuta

Sara Elizabeth Heisel

Nancy M Houmard

Jianping Xu

Thomas J Larosa

Brian J Martinell

Qi Wang

Kenneth A Barton

Yong Huang

Thomas J LaRosa

Kenneth John Piller

Toni Armstrong

Laura J Crow

Jon Lamb

Jason Ward

Joanne J Fillatti

Toni Alols Voelker

Jeffrey M Staub

Wanggen Zhang

Susan Johnson

Tichafa R Munyikwa

Michael W Peterson

Artem G Evdokimov

John D Bradley

David N Walters

Yuanji Zhang

Linda L Lutfiyya

Victor M Guzov

Liang Guo

Michael H Luethy

Ervin D Nagy

David McElroy

Philip W Miller

Allen T Christian

Mingqi Deng

R Douglas Sammons

Alessandra Frizzi

Brian Edward Weiner

David Walters

G David Grothaus

Qing Huai

Timothy K Ball

Christine Shyu

Peizhen Yang

Ryan K Bartlett

Sonya J Franklin

Phillip W Miller

Peter H J Hajdukiewicz

Parthasarathy Ramaseshadri

Byron V Olsen

Yifei Kong

Melissa M Kemp

Jeffrey A Haas

Elysia Krieger

Tichafa Ri Munyikwa

Edwards Allen

Sergey I Ivashuta

James K Roberts

Sara E Heisel

David E Kovalic

Yaunji Zhang

Michael H Leuthy

Xudong Xe

Shinhshieh Huang

Kenneth Barton

The present disclosure relates to compositions and methods for improving site-directed integration frequency in the genome of plant cells.

Methods to improve site-directed integration frequency

The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

Compositions and methods for control of insect infestations in plants

Pesticidal proteins are provided that combine two or more modes of action into a single molecule. These pesticidal proteins therefor exhibit greater efficacy and/or durability of resistance, for the purposes of pest control, and can be utilized for pest control by provision in the diet of a pest organism, or by topical application to crop plants and/or pests. Methods and compositions for producing and using such proteins are also provided.

Multi functional toxins

Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

Methods and compositions for expression of transgenes in plants

The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

DNA constructs for obtaining marker-free transgenic plants

Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.

Recombinant DNA constructs employing site-specific recombination

This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.

Methods for producing hybrid seed

The present invention relates to methods and compositions for improving the efficiency of- and-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

Use of multiple transformation enhancer sequences to improve plant transformation efficiency

The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

Recombinant DNA constructs and methods for controlling gene expression

Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

Vectors and methods for improved plant transformation efficiency

Growing community of inventors

Chesterfield, MO, United States of America

Larry A Gilbertson