Ajinomoto Co., Ltd.

Larisa Gotlibovna Airikh

Vera Georgievna Doroshenko

Elena Vitalievna Klyachko

Mikhail Markovich Gusyatiner

Yoshihiko Hara

Elvira Borisovna Voroshilova

Yury Ivanovich Kozlov

Konstantin Vyacheslavovich Rybak

Takuji Ueda

Tatyana Viktorovna Leonova

Sergei Vladimirovich Mashko

Ekaterina Aleksandrovna Slivinskaya

Ekaterina Alekseevna Savrasova

Valeriy Zavenovich Akhverdian

Rustem Saidovich Shakulov

Akira Imaizumi

Irina Sergeevna Tsyrenzhapova

A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3'-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.

Method for producing lower alkyl ester

A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of thefamily, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3'-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.

L-amino acid producing bacterium

There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

Method for producing L-amino acids using bacteria of the enterobacteriaceae family

A method is provided for producing an L-amino acid by culturing a microorganism belonging to the Enterobacteriaceae family and having the ability to produce an L-amino acid, in a medium to produce and accumulate the L-amino acid in the medium. The microorganism has been modified by introduction of a DNA fragment which includes a pho regulon promoter and a structural gene encoding an L-amino acid biosynthetic enzyme, which is ligated downstream of the promoter so that the gene is expressed by the promoter, and so that the activity of the L-amino acid biosynthetic enzyme is increased by the expression of the gene by the promoter. In this way, the L-amino acid that is produced in the medium can be collected. Furthermore, the phosphorus concentration in the medium is such that the expression of the gene by the promoter is induced.

Method for production of an L-amino acid

Method for producing L-amino acids using bacteria of the Enterobacteriaceae family

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Moscow, Russia

Larisa Gotlibovna Airikh