Other

North Carolina State University

Washington State University

The United States of America As Represented by the United States

University of Florida Research Foundation, Incorporated

The United States of America As Represented by the Secretary of

Arizona Board of Regents, a Body Corporate

Lance E Perryman

Travis C McGuire

Michael W Riggs

William C Davis

Terry F McElwain

Douglas P Jasmer

Donald P Knowles, Jr

Guy H Palmer

Will L Goff

Stephen A Hines

David W Reduker

DNA sequences encoding epitopes to which sporozoite-neutralizing antibodies are directed are provided. Recombinant proteins and synthetic peptides containing epitopes for inducing an antigenic response are described.

Neutralization-sensitive epitopes of Cryptosporidium parvum

Compositions and methods useful for conferring passive or active immunity to the parasite, . A high molecular weight glycoprotein antigen isolated from , capable of binding the mAb 3E2, was shown to harbor an epitope critical for triggering the neutralizing CSP-like reaction in the parasite. Antibodies targeted against the critical epitope were shown to possess neutralizing activity, and could be combined with other anti- monoclonal antibodies and administered to an animal to confer passive immunity. Immunogenic compositions including the purified antigen are disclosed for use in stimulating an active immune response against

Anti-cryptosporidium parvum preparations

DNA sequences encoding epitopes to which sporozoite-neutralizing antibodies are directed are provided. Recombinant proteins and synthetic peptides containing,epitopes for inducing, an antigenic response are described.

Compositions and methods useful for conferring passive or active immunity to the parasite, C. parvum. A high molecular weight glycoprotein antigen isolated from C. parvum, capable of binding the mAb 3E2, was shown to harbor an epitope critical for triggering the neutralizing CSP-like reaction in the parasite. Antibodies targeted against the critical epitope were shown to possess neutralizing activity, and could be combined with other anti-C. parvum monoclonal antibodies and administered to an animal to confer passive immunity. Immunogenic compositions including the purified antigen are disclosed for use in stimulating an active immune response against C. parvum.

Anti-Cryptosporidium parvum preparations

The present invention relates to a purified and isolated merozoite protein which is a specific indicator of infection by Babesia equi (B. equi) in horses. This protein contains a conserved region found in all strains of B. equi. It has a molecular weight of approximately 28 KDa and has been successfully purified and sequenced. The isolated and purified merozoite protein is used to prepare antibodies which can then be used in a competitive inhibition enzyme linked immunosorbent assay for the diagnosis of B. equi infection in horses.

Merozoite proteins for use in detection of Babesia equi in horses using

Antigenic surface proteins from the intraerythrocytic merozoite stage of Babesia bigemina have been isolated using cell fusions and monoclonal antibodies produced thereby. The gene encoding a 58 kD surface protein has been identified and the DNA sequence determined and compared with sequences of other known merozoite proteins. Immunization of mammals, such as bovines, with purified isolates induces an immunological response that is effective to reduce pathological effects of babesiosis induced by Babesia bigemina. Diagnostic kits using monoclonal antibodies and antigenic surface proteins of Babesia bigemina are also disclosed.

Immunization against babesiosis using purified surface antigens of

Antigenic surface proteins from the intraerythrocytic merozoite stage of Babesia bigemina have been isolated using cell fusions and monoclonal antibodies produced thereby. Immunization of mammals, such as bovines, with purified isolates induces an immunological response that is effective to reduce pathological effects of babesiosis induced by Babesia bigemina. Diagnostic kits using monoclonal antibodies and antigenic surface proteins of Babesia bigemina are also disclosed.

Immunization against Babesiosis using purified merozoite surface

The subject invention concerns the identification of novel merozoite surface proteins of Babesia bovis. Also disclosed are monoclonal antibodies to these proteins as well as genes which encode for the proteins. The invention further concerns the use of the novel proteins, recombinant DNA clones, and monoclonal antibodies in the detection, treatment, and prophylaxis of babesiosis.

Growing community of inventors

Cary, NC, United States of America

Lance E Perryman