Harvard College

Children's Medical Center Corporation

The General Hospital Corporation

Lance Davidow

Lee L Rubin

Stuart L Schreiber

Douglas A Melton

Derrick J Rossi

Morag Helen Stewart

Shuibing C Chen

Malgorzata Borowiak

Wataru Ebina

Lee F Peng

Paula Gutierrez-Martinez

Kelvin Lam

Julia Lamenzo Fox

Julia Lamenzo

The methods and compositions described herein relate to producing, expanding, enriching, and/or maintaining hematopoietic stem cells ex vivo by treating the cells with an agent(s) that exhibits two or more activities selected from modulation of histone methylation; inhibition of TGFβ signaling; inhibition of p38 signaling; activation of canonical Wnt signaling; and modulation of histone acetylation. In some embodiments, the technology described herein relates to transplantation of hematopoietic stem cells.

Methods and compositions relating to hematopoietic stem cell expansion, enrichment, and maintenance

The methods and compositions described herein relate to producing, expanding, enriching, and/or maintaining hematopoietic stem cells ex vivo by treating the cells with an agent(s) that exhibits two or more activities selected from modulation of histone methylation; inhibition of TGF&#x3b2; signaling; inhibition of p38 signaling; activation of canonical Wnt signaling; and modulation of histone acetylation. In some embodiments, the technology described herein relates to transplantation of hematopoietic stem cells.

Certain embodiments disclosed herein are directed to a method of producing pancreatic cells or pancreatic cell precursors by exposing human embryonic stem cells to an effective amount of at least one compound listed in Table I to differentiate the human embryonic stem cells into the pancreatic cells or the pancreatic cell precursors. Kits and pancreatic cell lines produced using the methods are also described.

Compositions and methods for promoting the generation of PDX1+ pancreatic cells

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Lexington, MA, United States of America

Lance Davidow