Syngenta Mogen B.v.

Zeneca Mogen B.v.

Lambertus Henricus Simons

Maarten Hendrik Stuiver

Jer&ocirc;me Hubertina Henricus Victor Custers

Anne Silene Ponstein

Stephan Andreas Ohl

Oscar Johanna Maria Goddijn

Nicolas Elzinga

Bernardus Martinus Maria Dekker

Sietske Hoekstra

Hendrik Tigelaar

The invention provides novel Ti-plasmid vectors for-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that reduces or eliminates the occurrence of transformants with vector read-through DNA sequence. The invention also provides methods of plant cell transformation utilizing these novel vectors.

Plasmids for plant transformation and method for using the same

The invention comprises a method for the induction of pathogen resistance in plants characterized by transforming a plant with a polynucleotide sequence comprising a pathogen inducible promoter which regulates the expression of a plant signal transduction protein or a homologue thereof which when constitutively expressed gives rise to a hypersensitive response in plants. Alternatively, the invention comprises a method for the induction of pathogen resistance in plants characterized by transforming a plant with a polynucleotide sequence comprising a pathogen inducible promoter which regulates the expression of a compound which is able to alleviate the inhibitory effect of a protein on the signal transduction pathway leading to a hypersensitive response in plants.

Method for the induction of pathogen resistance in plants

This invention describes pathogen-inducible promoters which normally drive expression of plant hexose oxidases, especially those which can be isolated from and , more specifically those promoters which naturally are the regulatory regions driving expression of the hexose oxidase MS59 and WL64, respectively. Also claimed are chimeric constructs where these pathogen-inducible promoters drive expression of antipathogenic proteins or of proteins which can elicit a hypersensitive response.

Pathogen-inducible promoter

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Amstelveen, Netherlands

Lambertus Henricus Simons