General Electric Company

Beckman Coulter, Inc.

Ge Healthcare Bio-sciences Corp.

Leica Microsystems Cms Gmbh

Global Life Sciences Solutions USA LLC

Lakshmi Sireesha Kaanumalle

Arunkumar Natarajan

Anup Sood

Kwok Pong Chan

Christina Lowes

Hashem Akhavan-Tafti

Kevin Bernard Kenny

Ravinder K Gupta

Renuka De Silva

Robert A Eickholt

Guoping Wang

Elizabeth Mary McDonough

John Richard Nelson

Robert John Filkins

Christopher Michael Puleo

John Frederick Graf

Victoria Eugenia Cotero

Kashan Ali Shaikh

Ralf Lenigk

Abraham Joy

Dmitry Vladimirovich Dylov

Patrick McCoy Spooner

Wei Gao

Nicholas Nun

The invention discloses a method for reduction of autofluorescence in biological samples, comprising the steps of:

Method for reduction of autofluorescence from biological samples

The subject matter of the present disclosure generally relates to techniques for following a treatment protocol having one or more treatment parameters to cause a targeted physiological outcome at a distal site, assessing an expression level of a gene in a region of interest after completing the treatment protocol, and modifying the one or more treatment parameters based on the expression level of the gene. The treatment protocol may include one or more ultrasound energy treatments to the region of interest.

Neuromodulation techniques

Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of binding at least one probe to one or more target present in a biological sample including multiple targets, and detecting a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with a photoinitiator and irradiating the sample, thereby initiating a photoactivated chemical bleaching process that substantially inactivates the probe. The method further includes the steps of binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The process of binding, detecting and bleaching may be iteratively repeated.

Chemical bleaching of dyes using radical photoinitiators

A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.

Photodegradable sample collection system and method

Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample including multiple targets, binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with an electron transfer reagent, as well as an optional additive which prevents target modification during photoactivated chemical bleaching, and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The process of binding, defecting and bleaching may be iteratively repeated.

Photoactivated chemical bleaching of dyes

Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with a cationic or zwitterionic borate compound and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The process of binding, observing and bleaching may be iteratively repeated.

Photoactivated chemical bleaching of dyes using borates

Methods comprising probing multiple targets in an H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, optionally detecting H&E staining of the sample, removing the hematoxylin and eosin signals, and detecting additional features or targets in the biological sample. The detecting step may include performing the steps of binding at least one probe to one or more targets to the sample, detecting a signal from the probe and contacting the sample with a bleaching agent. The process of binding, detecting and bleaching may be iteratively repeated.

Methods of analyzing an H and E stained biological sample

A borate compound for use as photoinduced chemical bleaching reagent is disclosed having improved bleaching performance under certain conditions, compared to neutral borates. The compounds are comprised of Formula I;RRRRBX&#x2003;&#x2003; Iwhere each R, R, and Rand Ris, independently, an alkyl, alkylaryl, or arylalkyl, with the proviso that at least one of R, R, and Rand Ris an alkyl group and at least two of R, R, Rand Rgroup is positively charged; X is an anion; and a equals 1, 2, or 3.

Borates for photoactivated chemical bleaching

Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with an electron transfer reagent and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The process of binding, observing and bleaching may be iteratively repeated.

Methods comprising the use probing multiple targets in a H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, observing the sample, removing the hematoxylin and partially removing the eosin by washing the sample, contacting the sample with a borate salt, and irradiating the sample to remove the residual eosin fluorescence. The method further includes the optionally performing the additional steps of binding at least one probe to one or more targets to the sample, observing a signal from the probe and contacting the sample with a bleaching agent. The process of binding, observing and bleaching may be iteratively repeated.

The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.

Growing community of inventors

Niskayuna, NY, United States of America

Lakshmi Sireesha Kaanumalle