Dow Agrosciences LLC

Corteva Agriscience LLC

Sangamo Therapeutics, Inc.

Sangamo Biosciences, Inc.

Lakshmi Sastry-Dent

Zehui Cao

Steven Robert Webb

Shreedharan Sriram

Debra L Camper

W Michael Ainley

Fyodor Urnov

Manju Gupta

Ning Zhou

Dmitry Y Guschin

Jayakumar Pon Samuel

Paul Bundock

Vipula Shukla

Michiel Jan De Both

Joseph F Petolino

Navin Elango

Steven Lee Evans

Wei Chen

Liu Shen

Cristie Min Dewes

Matthew A Simpson

David Richard Corbin

James Wayne Bing

Gregory J Cost

William M Ainley

Ryan C Blue

Steven Arnold Thompson

Mary E Welter

Pohao Wang

Cristie M Dewes

Liu Y Shen

Michael W Ainley

Steven L Evans

As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

Optimal soybean loci

As disclosed herein, optimal native genomic loci from maize plants have been identified that represent best sites for targeted insertion of exogenous sequences.

Optimal maize loci

As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

As disclosed herein, optimal native genomic loci have been identified in monocot plants, such as maize plants, that represent best sites for targeted insertion of exogenous sequences.

Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.

Targeted modification of malate dehydrogenase

The present invention is based on the use of the H3K9me2 methylation levels in plant genomes to predict transgene silencing, transgene stability, and/or transgene expression level. Provided are methods and/or systems for generating whole-genome H3K9me2 maps and its use with an assigned threshold value for predicting gene silencing. The methods and/or systems provided herein can be used in high-throughput setting for screening large number of transformed event in a relatively short period of time as compared to existing technologies.

Methods and systems for predicting the risk of transgene silencing

The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Precision gene targeting to a particular locus in maize

The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

DNA detection methods for site specific nuclease activity

Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.

Methods and compositions for nuclease-mediated targeted integration of transgenes

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Avon, IN, United States of America

Lakshmi Sastry-Dent