Oxford Nanopore Technologies Limited

Vib Vzw

Vrije Universiteit Brussel

Ohio State Innovation Foundation

Katholieke Universiteit Leuven

Lakmal Nishantha Jayasinghe

Elizabeth Jayne Wallace

Andrew John Heron

James Anthony Clarke

Mark Bruce

James White

Richard George Hambley

Rebecca Victoria Bowen

Luke McNeill

Neil Roger Wood

Pratik Raj Singh

Jonathan Bankes Pugh

Szabolcs Soeroes

Han Karel Remaut

Domenico Caprotti

John Milton

Clive Gavin Brown

John Hagan Pryce Bayley

Ruth Moysey

Christopher Peter Youd

Ramiz Iqbal Nathani

Michael Robert Jordan

Brian McKeown

David Alves

Stephen Cheley

John Joseph Kilgour

Stephen Robert Young

Daniel John Turner

Joseph Hargreaves Lloyd

Sander Egbert Van Der Verren

Christopher James Wright

Paul Richard Moody

Peixuan Guo

Hagan Bayley

Daniel Ryan Garalde

Stefan Howorka

Nani Van Gerven

David Jackson Stoddart

Richard Muscat

Luke Mcneill

Jayne Wallace

Giovanni Maglia

Terence Alan Reid

Mihaela Misca

Antonino Puglisi

Jessica Mary May Knott

Anthony Clarke

Jessica Mary May Johnson

Zhouxiang Ji

Lorenzo Franceschini

Tine Brouns

Mark John Bruce

Han Remaut

David Antoni Alves

Brian Mckeown

The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.

Mutant pore

The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.

Mutant CsgG pores

The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.

Mutant lysenin pores

The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Modified helicases

The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.

Mutant pores

The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.

Method for nanopore RNA characterization

The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.

Protein pores

Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.

Transmembrane pore consisting of two CsgG pores

The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.

Disclosed herein are compositions and methods that involve inserting connector protein channels of bacteriophage DNA packaging motors into copolymeric membranes via liposome-polymer fusion, which can be used as nanopore sensors for biomedical applications such as high throughput protein sequencing or cancer diagnosis. For example, disclosed are compositions comprising a copolymeric membrane into which a connector protein channel of a bacteriophage packaging motor has been inserted.

Bacteriophage-derived nanopore sensors

The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Modified enzymes

Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Method of characterizing a polynucleotide

The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

Enzyme-pore constructs

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Oxford, United Kingdom

Lakmal Nishantha Jayasinghe