University of Iowa Research Foundation

Lacy Daniels

John P Rosazza

Tao Li

Aimin He

Robert J Linhardt

Wilhelm Pickenhagen

Ian Fotheringham

Friedrich Mayerl

Ian Fotherington

Barbara A Bryan

The invention provides the nucleotide sequence and amino acid sequence for the enzyme carboxylic acid reductase isolated from bacteria. Expression cassettes, vectors, transformed cells, and variants are also provided as methods for use of recombinant biocatalytic reagents in production of synthetic, aromatic, aliphatic and alicyclic aldehydes and alcohols.

Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use

A method for large-scale production of rhamnose and 3-hydroxydecanoic acid is disclosed comprising the steps of growing microorganisms of Pseudomonas sp. capable of production of high levels of rhamnolipid in a defined culture medium containing vegetable oil. Additional steps include isolating the rhamnolipid from the culture medium, hydrolyzing the rhamnolipid to produce rhamnose and 3-hydroxydecanoic acid, and separating the rhamnose from the acid. Corn oil is the preferred vegetable oil and Pseudomonas aeruginosa is the preferred Pseudomonas sp. Non-limiting concentrations of nitrogen compounds and magnesium compounds and limiting concentrations of iron compounds are additionally preferably included in the culture medium.

Method for producing rhamnose

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Iowa City, IA, United States of America

Lacy Daniels