Cognis Corporation

Other

Cognis IP Management Gmbh

L Dudley Eirich

David L Craft

C Ron Wilson

Martin Anthony Gleeson

Maria Tang

Krishna M Madduri

John C Loper

Mark Eshoo

Cathy A Cornett

Alfred A Brenner

Manfred Biermann

Kevin W Anderson

Jeffrey A Gates

Gilbert H Vice

A bioprocess for producing carboxylic acids, alcohols and aldehydes is provided by culturingsp. in a fermentation medium containing various defined substrates.

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The present invention provides fatty alcohol oxidase (FAO) proteins and nucleic acid molecules encoding the FAO proteins. Also provided are analogs, derivatives, and enzymatically active fragments of the FAO proteins. Vectors and host cells comprising the nucleic acid molecules encoding the FAO proteins, analogs, derivatives and enzymatically active fragments thereof are also provided. In addition, FAO signature peptides and isolated nucleic acid molecules encoding the signature peptides are provided by the present invention. Methods of producing or increasing production of a subject FAO protein, methods for increasing aldehyde production during the second step of the &#x3c9;-oxidation pathway of fatty acids, methods for increasing production of a ketone from an alcohol during the second step of the &#x3c9;-oxidation pathway of fatty acids, and methods for increasing production of a dicarboxylic acid are also provided.

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &#x3c9;-hydroxylase complex of20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &ohgr;-hydroxylase complex of,20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto

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Cincinnati, OH, United States of America

L Dudley Eirich