Amoco Corporation

Gene-trak Systems

Kyriaki Parodos

David J Lane

Ayoub Rashtchian

Janice McCarty

Janice M McCarty

David Sobell

David A Hendricks

Hsien-Yeh Hsu

Susan Rigby

Nucleic acid fragments capable of hybridizing to rRNA of a Salmonella species and not capable of hybridizing to rRNA of Escherichia coli.

Detection of salmonella

The invention relates to methods of detection of bacteria of the genus Shigella and/or Enteroinvasive E. coli (EIEC) by use of a set of nucleic acid probes. The invention further relates to a set of Shigella specific chromosomal sequences and fragments and to probes derived from the Shigella specific fragments. Additionally, probes were derived from a sequence from the Shigella ompA gene. In particular, a series of probes, each approximately 40 nucleotides in length, were designed having specificity for Shigella or for Shigella and Enteroinvasive E. coli, and having utility in non-isotopic test formats which require amplification to achieve high sensitivity. Specific hybridization probe sets which are capable of detecting substantially all clinically significant serotypes of Shigella, as well as enteroinvasive strains of E. coli, are disclosed.

Nucleic acid probes for the detection of shigella

A method for detecting and/or quantitating specific DNA and/or RNA transcripts of human papillomavirus (HPV) is described. Nucleic acid probes specific for DNA or transcripts are used to determine the amount of the DNA or transcripts, and to ascertain the degree of amplification of certain high-oncogenic HPV genes. The method provides an accurate and reliable method for prognosticating serious cervical neoplasias and cancers.

Detection of HPV transcripts

Oligonucleotide probes for detection of salmonella

Nucleic acid probes capable of specifically hybridizing to rRNA of E. coli and Shigella species and not to rRNA of non-E. coli/Shigella are described along with methods utilizing such probes for the specific detection of E. coli and/or Shigella in food and other samples.

Probes for the specific detection of Escherichia coli and Shigella

Growing community of inventors

Framingham, MA, United States of America

Kyriaki Parodos