Toyota Jidosha Kabushiki Kaisha

Kyozo Ogura

Tanetoshi Koyama

Ayumi Koike

Shusei Obata

Masayoshi Muramatsu

Yenwin Cho

Naoto Shimizu

Tokuzo Nishino

Shinichi Ohnuma

Ayumi Takeshita

Takeshi Nakazawa

The present invention provides a polypeptide selected from a polypeptide of subunit (A) or a polypeptide of subunit (B) of a prenyl diphosphate synthetase; a DNA coding for the polypeptide; a recombinant vector comprising the DNA; a transformant transformed with the vector; and a method for preparing an active type prenyl diphosphate synthetase. Also disclosed is a method for preparing an active type enzyme on which a specific property has been conferred, comprising mixing polypeptides of the two subunits of a heterodimeric enzyme, the polypeptides being derived from different organisms and one of the polypeptides having the specific property.

Prenyl diphosphate synthetase genes

Method of making recombinant enzyme

Heptaprenyl diphosphate (HDP)-synthetase derived from Bacillus stearothermophilus which enzymes have the amino acid sequences shown as SEQ ID NOs: 1 to 3; 1 and 2; 2 and 3; or 1 and 3, DNA encoding them, and a method of producing the enzymes. According to the invention it is possible to industrially produce HDP-synthesizing enzyme and HPD.

Heptaprenyl diphosphate-synthetase

A DNA sequence that encodes a stable farnesyl pyrophosphate synthetase and the use thereof are provided. DNA sequence that encodes a farnesyl pyrophosphate synthetase originating from Bacillus stearothermophilus is provided. By selecting Bacillus stearothermophilus as the gene origin of the synthetase, a production system for the synthetase, a production system for the synthetase particularly having thermal stability, can be constructed.

Farnesyl pyrophosphate synthetase and DNA sequence encoding the same

DNA encoding heptaprenyl diphosphate synthetase

A mutated farnesyldiphosphate synthase capable of synthesizing geranylgeranyldiphosphate and gene coding for said mutated enzyme, wherein the mutated enzyme is modified from a native farnesyldiphosphate synthase by mutation of a gene coding for a native farnesyldiphosphate synthase.

Mutated farnesyldiphoshate synthase capable of synthesizing

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Sendai, Japan

Kyozo Ogura