Hitachi, Ltd.

Kyoko Makiguchi

Yasushi Nomura

Hisayuki Sagusa

Masumi Suzuki

A kit for simultaneous immunoassay of at least two items which comprises a development layer material comprising a development layer permitting development of a sample; at least two, preferably three or more, reagents supported in optional places on the development layer and individually containing an antibody or antigen different from those of the other reagents; and a concave sample-spotting place provided in the same place as one of the places where the aforesaid reagents are supported or in a place remote from all of these places. And a process for simultaneous immunoassay of at least two items which comprising utilizing said kit. The reagents supported in the above mentioned optional places are contained in microcapsules or liposomes.

Immunoassay and measurement kit used therefor

A turntable holding a train of reaction containers thereon is continuously turned by one revolution plus an angle for a share of one container and then stopped. This makes one cycle. On the route of transfer of the reaction container, there are provided a light irradiation position and a light collection position. The reaction container into which a sample and a marker reagent have been introduced is transferred such that it does not stop at the irradiation position and the light collection position. The reaction container to which a monochromatic light for excitation has been irradiated at the irradiation position is transferred toward the light collection position. Fluorescence coming from the light collection position is lead to a photodetector, and the concentration of the target substance in the sample is determined from the detected fluorescence after arithmetic processing. Since the light collection position is provided at a place different from the irradiation position in the present apparatus, it is possible to measure fluorescence originating from the sample which is substantially free from the influence of background fluorescence.

Analytical method and apparatus for determining fluorescence or

An automated analyzing apparatus with a photometer including an entrance slit permitting passing therethrough of the entirety or a portion of a convergent light beam transmitted successively through a plurality of sample measurement cells each containing a sample solution therein and a sensor converting the quantity of light passed through the entrance slit into a physical quantity which is an objective of measurement. A scattered light sensor is disposed between the sample measurement cells and the entrance slit at a position where the sensor is not irradiated with a straight advancing component of the light beam, whereby measurement of immunity-related tests on the basis of the intensity of scattered light can be made together with measurement of biochemical tests on the basis of the intensity of transmitted light.

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Katsuta, Japan

Kyoko Makiguchi