Ajinomoto Co., Ltd.

Kyoko Koibuchi

Katsuhiko Kitamoto

Hiroaki Nagasaki

Jiro Kataoka

Ari Yuasa

Yoichi Ueda

Jun-ichi Maruyama

Daiki Ninomiya

Mari Kojima

Glutaminase is purified from an, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene fromgenomic library and cDNA library, andgenomic library by hybridization using the partial sequence as a probe.

Glutaminase, its gene and a method of producing it

The object of the present invention is to provide Koji mold aminopeptidases capable of efficiently hydrolyzing persistent peptides and also genes encoding the aminopeptidases. The present invention providesaminopeptidase and nucleic acid molecules encoding it. In particular, the present invention provides a protein having an amino acid sequence represented by amino acid Nos. 1 to 519 in SEQ ID NO: 2, or a protein containing the substitution, deletion, insertion, addition or inversion of one or more amino acids in said sequence, and which protein has an activity of catalyzing the reaction for releasing an amino acid at an N-terminal of a peptide, and nucleic acid molecules encoding them.

Aminopeptidase and the genes thereof

Glutaminase is purified from an , its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene from genomic library and cDNA library, and genomic library hybridization using the partial sequence as a probe.

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Kawasaki, Japan

Kyoko Koibuchi