Toray Industries, Inc.

Kyohei Isobe

Katsushige Yamada

Kenji Kawamura

Masateru Ito

Kenji Sawai

Shingo Hiramatsu

Satoshi Sakami

Hideshi Yanase

Izumi Morita

Koji Kobayashi

Shiomi Watanabe

Kyungsu Na

Disclosed is a genetically modified microorganism into which a nucleic acid encoding an enzyme that catalyzes a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA is introduced. In the genetically modified microorganism, a nucleic acid encoding any one of the polypeptides described in (a) to (c) below is introduced or expression of the polypeptide is enhanced: (a) a polypeptide composed of an amino acid sequence represented by any one of SEQ ID NOs: 1 to 6 and 213, (b) a polypeptide composed of the same amino acid sequence as any one of the amino acid sequences, except that one or several amino acids are substituted, deleted, inserted, and/or added, and having enzymatic activity that catalyzes a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, and (c) a polypeptide composed of an amino acid sequence with a sequence identity of not less than 70% to any one of the amino acid sequences and having activity to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.

Gene-modified microorganism for producing 3-hydroxyadipic acid, alpha-hydromuconic acid, and/or adipic acid, and production method for said chemical products

A method of producing 3-oxoadipic acid from an aliphatic compound easily utilizable by a microorganism, such as a saccharide, by utilization of a metabolic pathway of the microorganism is disclosed. The method of producing 3-oxoadipic acid includes the step of culturing at least one type of microorganism having a capacity to produce 3-oxoadipic acid, selected from the group consisting of, for example, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, and microorganisms belonging to the genus

Method for producing 3-Oxoadipic acid

Provided is a method of producing &#x3b1;-hydromuconic acid, the method including the step of culturing a microorganism belonging to the genuscapable of producing &#x3b1;-hydromuconic acid.

Method for producing alpha-hydromuconic acid

A method of producing &#x3b1;-hydromuconic acid using a metabolic pathway of a microorganism is disclosed. The method of producing &#x3b1;-hydromuconic acid includes the step of culturing at least one type of microorganism having a capacity to produce &#x3b1;-hydromuconic acid, selected from the group consisting of microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, microorganisms belonging to the genus, and microorganisms belonging to the genus

Method for producing &#x3b1;-hydromuconic acid

Provided is a method of producing 3-hydroxyadipic acid, the method including the step of culturing a microorganism belonging to the genuscapable of producing 3-hydroxyadipic acid.

Method for producing 3-hydroxyadipic acid

A novel method of producing 3-hydroxyadipic acid using a metabolic pathway of a microorganism is disclosed. The method of producing 3-hydroxyadipic acid includes the step of culturing at least one type of microorganism having a capacity to produce 3-hydroxyadipic acid, selected from the group consisting of microorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusmicroorganisms belonging to the genusand microorganisms belonging to the genus

Method for producing 3-oxoadipic acid

A method of producing 2,3-butanediol includes subjecting a 2,3-butanediol culture liquid produced by microbial fermentation to nanofiltration membrane treatment and ion-exchange treatment (Step A), and then adding an alkaline substance and performing distillation (Step B).

Process for producing 2,3-butanediol

A method produces a chemical product by continuous fermentation including filtering a culture liquid of a microorganism(s) through a separation membrane, retaining unfiltered liquid in, or refluxing unfiltered liquid to, the culture liquid, adding a fermentation feedstock to the culture liquid, and recovering a product in the filtrate, wherein the microorganism(s) is/are a microorganism(s) that undergo(es) catabolite repression, and the fermentation feedstock comprises hexose and pentose.

Method of producing chemical substance

A method of producing 2,3-butanediol includes the steps of: culturing a microorganism belonging to the genusin a fermentation feedstock containing a carbon source (Step A); and purifying 2,3-butanediol from culture liquid obtained in this step (Step B). In this method, the carbon source contains pentose, and the microorganism belonging to the genushas a capacity to metabolize pentose.

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Kamakura, Japan

Kyohei Isobe