Life Technologies Corporation

Molecular Probes, Inc.

Harvard College

Other

Invitrogen Corporation

Pierce Biotechnology, Inc.

University of Minnesota

Kyle R Gee

Vladimir Martin

Hee Chol Kang

Aleksey Rukavishnikov

Brian Agnew

Richard P Haugland

Jixiang Liu

Aimei Chen

Yi-Zhen Hu

Zhenjun Diwu

Wenjun Zhou

Daniel Beacham

Iain Johnson

Robert Aggeler

Shih-Jung Huang

Upinder Singh

Schuyler Corry

Xin Wang

Jolene Ann Bradford

Timothy J Mitchison

Thomas H Steinberg

Adrian Salic

Yexin Wu

Wai-Yee Leung

Wayne Forrest Patton

Joseph M Beechem

Anilkumar R Kore

Dieter H Klaubert

Scott Grecian

Shanmugasundaram Muthian

Surbhi Desai

Shaheer Khan

Kathleen Free

Lawrence Greenfield

Gayle Buller

Scott Sweeney

Michael Wayne Olszowy

Fei Mao

David M Hagen

Bruce Branchaud

Marie Christine Nlend

Brian Lee Evans

Eric Welch

Michael O'Grady

James Stray

Mahendra Rao

Paul J Millard

Lai-Qiang Ying

Jeffrey Dzubay

Levente Egry

Michael Janes

Evan Antoulinakis

Brian Patch

Adam York

Peter Slade

Robert H Batchelor

Yue Ge

Scott Clarke

Bradley Dubbels

Kathleen Kihn

Wei-Chuan Sun

Quentin Low

Matthew Baker

Christopher Langsdorf

Peter Smalley

William Downey

Brian Filanoski

Yolanda Tennico

James Hirsch

Monica Tomaszewski

Uma Lakshmipathy

Robert A Aarhus

Rene Quintanilla

Timothy F Walseth

Charles M Brenner

Hon Cheung Lee

Martin Poot

Maura J Ford

Kapil Kumar

Hee-Chol Kang

Christina Pastula

Jiaxing Liu

Richard M Graeff

Jongtae Yang

Tamara Nyberg

Bhaskar Mandavilli

Courtenay Hart Kerndt

Penny Melquist

Nancy Ahnert

Schuyler B Corry

Michael Olszowy

Levente Ergy

Kapil Kumal

Maura Ford

Judith Berlier

Robert Batchelor

Jason Dallwig

Water-soluble, fluorescent particles and compositions, kits, and methods of making and using such particles are disclosed. Processes for preparing fluorescent particles and for controlling the size, polydispersity and optical properties of such particles also are provided.

Fluorescent particles

Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SiR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.

Silicon-substituted rhodamine dyes and dye conjugates

This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.

Compositions and methods for enhanced fluorescence

A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I:

Fluorogenic pH sensitive dyes and their method of use

According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.

Protein melt analysis using dipyrrometheneboron difluoride compounds

Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.

Methods and compositions for assessing cell viability

Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.

7-hydroxycoumarin-based cell-tracking reagents

Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

Compounds and methods for conjugation of biomolecules

Provided herein are compounds, enzyme substrates, compositions, kits, uses, and methods for detecting the presence or absence of a caspase enzyme, measuring the activity of a caspase enzyme, or detecting the presence or absence of apoptosis. The detection or measurement can occur through intracellular cleavage of a compound or enzyme substrate, which can lead to an increase in fluorescence, e.g., in the violet or red channel, through liberation of a nucleic acid binding dye from a peptide, such as liberation of a DNA-binding dye from a negatively charged peptide comprising a sequence recognized and cleaved by a caspase.

Methods and compositions for detecting or measuring caspases or apoptosis

A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.

The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

Hydrazinyl and aminooxy compounds and their methods of use

Described herein are fluorescent compounds and methods and comprising these compounds. The compounds disclosed herein are carbopyronine reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.

Carbopyronone compounds useful as diagnostic adjuvants

Growing community of inventors

Springfield, OR, United States of America

Kyle R Gee