Axys Pharmaceuticals, Inc.

Other

Kyle C Elrod

Thomas E Jenkins

James M Clark

Robert M Stroud

Bradley A Katz

William R Moore

Richard D Tanaka

The present invention relates to serine protease inhibitors having a P site binding moiety and a divalent cation(s) chelating moiety which are pre-prepared as divalent cation(s) complexes or combined with the serine protease in the presence of divalent cation(s) and methods for identifying and using the inhibitors. The compounds are shown to have high inhibitory activity when complexed to divalent cation(s) and are useful in various processes associated with serine protease isolation and inhibition.

Metal mediated serine protease inhibitors

A method is presented for determining if the inhibition of metal mediated serine proteases can be potentiated by cobalt or zinc ions.

Potentiating metal mediated serine protease inhibitors with cobalt or zinc ions

This invention provides methods of detecting mast cell mediated disease in an individual by detecting the presence in the individual of autoantibodies against mast cell specific proteins, such as tryptase and chymase. Specific mast cell mediated diseases detected by this invention include asthma, rheumatoid arthritis and SLE. The invention also provides a method of detecting autoantibodies against a mast cell specific protein. The method comprises exposing a sample to an sbp member complementary to the autoantibody and detecting the specific binding between the autoantibody and the sbp member. A related aspect of the invention provides methods of treating mast cell mediated diseases by monitoring for the presence of these autoantibodies. Another aspect of the invention relates to a composition comprising an autoantibody against a mast cell specific protein and the mast cell specific protein. A related aspect of the invention is to a method of forming a composition comprising an immune complex of a tryptase autoantibody and tryptase, wherein the complex is bound to a solid support.

Anti-tryptase detection as a diagnostic for inflammatory diseases

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Fremont, CA, United States of America

Kyle C Elrod