The University of Hong Kong

Versitech, Inc.

Other

Kwok Hung Chan

Kwok Yung Yuen

Yi Guan

Lit Man Poon

John M Nicholls

Joseph S M Peiris

Frederick C Leung

Kar Pui Susanna Lau

Chiu Yat Patrick Woo

Joseph Sriyal Malik Peiris

Feng Chen

Yi Tsun Kao

Leo Lit Man Poon

Jsm Peiris

The present invention relates to an isolated novel virus causing Severe Acute Respiratory Syndrome (SARS) in humans ('hSARS virus'). The hSARS virus is identified to be morphologically and phylogenetically similar to known member of Coronaviridae. The present invention provides the complete genomic sequence of the hSARS virus. Furthermore, the invention provides the nucleic acids and peptides encoded by and/or derived from the hSARS virus and their use in diagnostic methods and therapeutic methods, including vaccines. In addition, the invention provides chimeric or recombinant viruses encoded by said nucleotide sequences and antibodies immunospecific to the polypeptides encoded by the nucleotide sequences.

Human virus causing severe acute respiratory syndrome (SARS) and uses thereof

The present invention provides the complete genomic sequence of a novel human coronavirus, coined as coronavirus-HKU1 ('CoV-HKU1'), isolated in Hong Kong from a patient who had a recent history of visit to Schenzhen, China. The virus belongs to the order Nidovirales of the family Coronavirdae, being a single-stranded RNA virus of positive polarity. The invention also provides the deduced amino acid sequences of the complete genome of the CoV-HKU1. The nucleotide sequences and deduced amino acid sequences of the CoV-HKU1 are useful in preventing, diagnosing and/or treating the infection by CoV-HKU1. Furthermore, the invention provides immunogenic and vaccine preparations using recombinant and chimeric forms as well as subunits of the CoV-HKU1 based on the nucleotide sequences and deduced amino acid sequences of the CoV-HKU1.

Human virus causing respiratory tract infection and uses thereof

The present invention relates to a pharmaceutical composition useful for the treatment of diseases associated with viruses of the order Nidovirales of the family Coronaviradae, such as Severe Acute Respiratory Syndrome (SARS) viruses in humans and other animals. In particular, this invention relates to a naturally occurring compound, namely, baicalin, extracted and purified from the Chinese medicinal plantGeorgi (Chinese name: Huang Qin), that exhibits potent antiviral activity against members of the order Nidovirales of the family Coronaviradae that infects humans and other animals; in particular, SARS viruses in humans ('hSARS virus'). The invention also relates to a therapeutic method, using pharmaceutical compositions comprising baicalin compounds, for the treatment, amelioration, management or prevention of diseases associated with members of the order Nidovirales of the family Coronaviradae, such as hSARS.

Baicalin as a treatment for SARS infection

The present invention provides the complete genomic sequence of a novel human coronavirus, coined as coronavirus-HKU1 ('CoV-HKU1'), isolated in Hong Kong from a patient who had a recent history of visit to Schenzhen, China. The virus belongs to the order Nidovirales of the family Coronaviridae, being a single-stranded RNA virus of positive polarity. The invention also provides the deduced amino acid sequences of the complete genome of the CoV-HKU1. The nucleotide sequences and deduced amino acid sequences of the CoV-HKU1 are useful in preventing, diagnosing and/or treating the infection by CoV-HKU1. Furthermore, the invention provides immunogenic and vaccine preparations using recombinant and chimeric forms as well as subunits of the CoV-HKU1 based on the nucleotide sequences and deduced amino acid sequences of the CoV-HKU1.

The present invention relates to a high-throughput diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans ('hSARS virus'). In particular, the invention relates to a high-throughput reverse transcription-PCR diagnostic test for SARS associated(SARS-CoV). The present assay is a rapid, reliable assay which can be used for diagnosis and monitoring the spread of SARS and is based on the nucleotide sequences of the N (nucleocapsid)-gene of the hSARS virus. The present method eliminates false negative results and provides increased sensitivity for the assay. The invention also discloses the S (spike)-gene of the hSARS virus. The invention further relates to the deduced amino acid sequences of the N-gene and S-gene products of the hSARS virus and to the use of the N-gene and S-gene products in diagnostic methods. The invention further encompasses diagnostic assays and kits comprising antibodies generated against the N-gene or S-gene product.

High-throughput diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS)

The present invention provides the complete genomic sequence of a novel human coronavirus, coined as human coronavirus-HKU1 ('CoV-HKU1'), isolated in Hong Kong. The virus belongs to the order Nidovirales of the family Coronaviridae, being a single-stranded RNA virus of positive polarity. Further study on nasopharyngeal aspirates from patients with community-acquired pneumonia has revealed that there are two genotypes, genotype A and genotype B, for this virus. In addition to the genomic sequences of these two genotypes, the invention provides the deduced amino acid sequences of the complete genome of the CoV-HKU1. The nucleotide sequences and deduced amino acid sequences of the CoV-HKU1 are useful in preventing, diagnosing and/or treating the infection by CoV-HKU1. Furthermore, the invention provides immunogenic and vaccine preparations using recombinant and chimeric forms as well as subunits of the CoV-HKU1 based on the nucleotide sequences and deduced amino acid sequences of the CoV-HKU1.

The present invention relates to a diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans ('hSARS virus'). In particular, the invention relates to a real-time quantitative PCR assay for the detection of hSARS virus using reverse transcription and polymerase chain reaction. Specifically, the quantitative assay is a TaqMan&#xae; assay using the primers and probes constructed based on the genome of the hSARS virus. The invention further relates to a diagnostic kit that comprises nucleic acid molecules for the detection of the hSARS virus.

Diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS)

A light-up accessory which includes a plurality of elementary building units and a chaining arrangement, wherein each of the elementary building units includes a supporting case; a lighting system, which is disposed in the supporting case, and includes an illuminating unit; an operating system which includes an operating member electrically connected with the lighting system and operatively connected with an electrical power supply in such a manner that when the operating member is switched on, the illuminating unit is supplied with electrical power to illuminate. The chaining arrangement which contains a plurality of chaining passageways longitudinally formed on the supporting cases of the elementary building units respectively, and includes a connecting chain adapted to connect the elementary building units by passing through the chaining passageway of each of the elementary building units.

Light-up accessory

An automatic motor-output-redirection system includes an automatic motor-output-redirection mechanism and a motor control unit for controlling the automatic motor-output-redirection mechanism which includes a turntable base arranged to be driven to rotate by a motor assembly. A primary Output shaft is coupled with the motor assembly in rotatable movable manner. A second idle gear is rotatably supported on the turntable base to couple with the primary output shaft. A plurality of the planetary output shafts are coaxially supported by the casing at positions around the turntable assembly, wherein the primary output shaft is arranged to drive the second idle gear to move between an engagement position that one of the planetary output shafts is driven to rotate and a disengagement position that each of the planetary output shafts is in an idle manner.

Growing community of inventors

Hong Kong, China

Kwok Hung Chan