Roche Diagnostics Gmbh

Roche Molecular Systems, Inc.

Roche Diagnostics Operations, Inc.

Kurt Weindel

Christoph Seidel

Frank Bergmann

Peter Wenzig

Joachim Brand

Gerhard Lassonczyk

Herbert Von Der Eltz

Emad Sarofim

Thomas Walter

Ralf Zielenski

Michael Riedling

Albert Geiger

Hans Hornauer

Judith Pinsl-Ober

Ursula Giesen

Erich Kyger

Michael Kirchgesser

G&uuml;nter Ziegler

Ralf Schoenbrunner

Patrick O'Donnell

Khushbeer Malhotra

Knut Bartl

This invention relates to magnetic particles having a glass surface which are substantially spherical. This invention also relates to methods for making them, as well as to suspensions thereof and their uses for the purification of DNA or RNA in particular in automated processes.

Magnetic glass particles, method for their preparation and uses thereof

This invention relates to a method for the isolation of a target nucleic acid using a material with an unmodified silica surface and subsequent amplification of the target nucleic acid in the presence of the material with an unmodified silica surface. The method is preferably carried out as an automated process preferably in a high throughput format. The method is preferably used in diagnostics.

Use of silica material in an amplification reaction

The present invention is related to compounds comprising mannitol or glucitol derivatives which may be used to build up oligomeric compounds. The invention is further related to uses of these oligomeric compounds for hybridization and as probes. In addition, methods for the detection of nucleic acids are disclosed wherein the oligomeric compounds are used.

Mannitol and glucitol derivatives

A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; (b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.

Methods for isolating nucleic acids

The discriminating capability of hybridization assays is increased by a combination of labelled primers which produce amplificates of one strand of a nucleic acid with a capture probe which is complementary to the same strand of the nucleic acid.

DNA detection by means of a strand reassociation complex

The invention concerns a method of detecting a substance to be analyzed in a sample by contacting the sample with a probe which contains nucleobases and two or more non-nucleosidic marker-promoting groups in conditions in which the substance to be analyzed binds indirectly or directly to the probe. The method enables the binding product to be detected. The probe is preferably branched.

Method of detecting a substance to be analyzed

The combination of two mechanisms of detection in a single device yields a method which allows the simple quantification of analyte determinations. Suitable formats and reagent kits are suggested.

Determination of analytes by means of two markers

The present invention concerns a method for the detection of an analyte in a sample liquid by luminescence measurement according to the principle of a ligand-receptor assay, e.g. an immunoassay or a hybridization assay or a combination thereof, wherein a sample liquid is incubated with at least one receptor which carries a luminescent label and the presence or/and the amount of the analyte to be detected is determined in the sample liquid by measuring the luminescence in a measuring medium containing dispersed components.

White trigger preparations for improving the signal detection in bioluminescent and chemiluminescent reactions

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Wielenbach, Germany

Kurt Weindel