Dpx Holdings B.v.

Upfront Chromatography A/s

Kurt Hauge Jensen

Allan Otto Lihme

Rene Oehlenschlaeger Holte

René Oehlenschläger Holte

Tony Christensen

In the field of expanded bed adsorption chromatography, with particular but not exclusive relevance to disposable expanded bed chromatography columns, a method of conducting upward flow expanded bed chromatography comprising: supplying a liquid via an inlet to a stationary phase medium contained in a column, allowing adsorption of at least one component from the liquid by the stationary phase medium, withdrawing the liquid from the column via an outlet, regulating the expansion of the stationary phase medium by regulation of the flow rate of the liquid through at least the inlet, and restricting any overpressure in the headspace of the column to not more than the outside pressure plus 0.1 bar, is provided. In addition, apparatus for use in said method, in particular columns for use in expanded bed chromatography, are provided.

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Roskilde, Denmark

Kurt Hauge Jensen