Mitsui Toatsu Chemicals, Incorporated

Kunizou Mori

Nobuhiro Kawashima

Noriko Morii

Katsuyuki Suzuki

Toshihiko Suzuki

Masaharu Ohoka

Mitsuyoshi Morii

Tissue plasminogen activator (tPA) species having a specific molecular weight can be isolated in a purified form from a crude tPA preparation containing various tPA species having different molecular weights by bringing the crude tPA preparation into contact with hydroxyapatite and then separately eluting the adsorbed tPA species with eluents having different pHs and/or salt concentrations.

Purification procedure of TPA from crude preparations

Tissue plasminogen activator (tPA) species having a molecular weight of about 70,000 daltons is isolated in a purified form from a crude tPA preparation containing various tPA species having different molecular weights by bringing the crude tPA preparation into contact with a cation exchanger in advance and separating the desired tPA species selectively from the cation exchanger by means of the salt gradient elution method.

Process of purifying tPA

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Yokohama, Japan

Kunizou Mori