Tokyo Electron Limited

Sinfonia Technology Co., Ltd.

Shimadzu Corporation

Kunitada Hatabayashi

Takashi Suzuki

Kenichi Kagawa

Shigenori Ozaki

Kiyoshi Mori

Yasuhiro Oshima

Hirotsugu Shiraiwa

Yusuke Yoda

Toshifumi Kitahara

Shinichi Gomi

Tomoaki Kurakazu

Kentaro Tomita

Daisuke Hiramaru

Masatoshi Takahashi

The present disclosure provides a method for determining the undifferentiated state of pluripotent stem cells, comprising: irradiating a test culture medium in which pluripotent stem cells are cultured with wavelength light having a wavelength in the range of 190 nm to 2,500 nm or a partial range thereof; detecting the reflected light, transmitted light or transmitted reflected light thereof to obtain absorbance spectrum data; and analyzing the absorbance at all or part of the measurement wavelengths in the absorbance spectrum data to determine the undifferentiated state of the pluripotent stem cells.

Method for determining undifferentiated state of pluripotent stem cell, method for subculturing pluripotent stem cell, and device for use in the methods

A culture container linkage device according to the present disclosure includes a first actuator configured to advance or retract needles, an actuator holder rotatably provided on a frame and configured to hold the first actuator, a second actuator, and a washer configured to wash the needles. The second actuator rotates the needles via the actuator holder. The needles are configured to be positioned, by the second actuator, at a container-facing position at which the needles face a culture container and a washing-facing position at which the needles face the washer.

Culture container linkage device, culture system, and method for washing needle

There is provided a method for easily determining an undifferentiated state of pluripotent stem cells without relying on the judgment of a skilled technician. The method includes: a step of evaluating an undifferentiated state of pluripotent stem cells based on a time-dependent change in a variation value of an extracellular metabolite contained in a culture medium in which the pluripotent stem cells are cultured, wherein the extracellular metabolite is at least one selected from a group consisting of L-glutamic acid, L-alanine and ammonia.

Method for determining undifferentiated state of pluripotent stem cells by culture medium analysis

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Tokyo, Japan

Kunitada Hatabayashi