Ajinomoto Co., Ltd.

Bridgestone Corporation

Kunio Nakata

Hiroki Yamaguchi

Masayuki Sugiki

Yoshinori Tajima

Yasuo Fukushima

Keiichi Yokoyama

Uno Tagami

Yosuke Nishio

Yoko Mihara

Saaya Takaoka

Takashi Oku

Yasuhiro Kashima

Yasuyuki Hayashi

Minako Harada

The present invention provides a mutated histidine decarboxylase suitable for a practical use. Specifically, the present invention provides a mutated histidine decarboxylase having at least one amino acid residue mutated as compared to a wild-type histidine decarboxylase, and having higher histidine decarboxylase activity and/or stability than the wild-type histidine decarboxylase, and also a use thereof. The mutated histidine decarboxylase has Motifs (1) to (6), and an amino acid residue in at least one motif thereof can be mutated. The mutated histidine decarboxylase can also have a mutation of at least one amino acid residue in an amino acid sequence designated by SEQ ID NO: 3 and in a homologous sequence thereto.

Mutated histidine decarboxylase and use thereof

The present application relates to a modified isoprene synthase that has an isoprene synthetic activity and has at least one mutation of an amino acid residue in the amino acid sequence of SEQ ID NO: 4, an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO: 4, or an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO: 4. The modified isoprene synthase is useful for preparing isoprene monomers in improved yields.

Modified isoprene synthase

The present invention provides means useful for establishing an excellent isoprene monomer production system. Specifically, the present invention provides a polynucleotide of the following (a), (b), or (c):

Isoprene synthase and gene encoding the same, and method for producing isoprene monomer

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Kawasaki, Japan

Kunio Nakata