Kyowa Hakko Kogyo Co., Ltd.

Kyowa Hakko Bio Co., Ltd.

Chisso Corporation

Waseda University

Api Corporation

T. Hasegawa Co., Ltd.

Tokai Bussan Co., Ltd.

Kuniki Kino

Masato Ikeda

Yoshiyuki Kuratsu

Tetsuya Abe

Kazuyuki Okamoto

Ryotaro Hara

Ryoichi Katsumata

Satoru Furukawa

Makoto Yagasaki

Yukinobu Kotani

Junichi Takano

Hiroshi Kawabata

Tetsuo Nakano

Toshiya Sawai

Ryoma Miyake

Kiyoji Hattori

Toshiki Furuya

Yasuhiro Tomiyoshi

Yuji Nakazawa

Kazuhiro Furukawa

Yasuya Takeda

Youichi Kotanaka

Yasuichiro Suga

Daisuke Koma

Junicho Takano

The present invention provides L-amino acid &#x3b1;-ligase represented by sequence number 1 (SEQ ID NO. 1), which is a mutant protein including substitution of at least 1-3 amino acid residues of amino acid sequence of protein YwfE, with the substitution being at least one of an asparagine (N) residue of the 108th place from the N terminal being substituted with at least one of an alanine (A) residue, a glutamic acid (E) residue and a glutamine (Q) residue, an isoleucine (I) residue of the 112th place from the N terminal being substituted with a valine (V) residue, and a histidine (H) residue of the 378th place from the N terminal being substituted with at least one of a lysine (K) residue or an arginine (R) residue.

Protein having synthetic activity for imidazole dipeptid and production method of imidazole dipeptide

The present invention provides a method of producing (&#x2212;)-rotundone from &#x3b1;-guaiene. The method includes the steps of: (1) allowing a cytochrome P450 protein to act on &#x3b1;-guaiene, which cytochrome P450 belongs to the CYP152 family and is capable of oxidizing the methylene group at position 3 of &#x3b1;-guaiene to the carbonyl group; and/or (2) allowing a cytochrome P450 protein to act on &#x3b1;-guaiene in the presence of an electron transfer protein capable of transferring electrons to the cytochrome P450 protein, which cytochrome P450 belongs to the CYP152, CYP106, or CYP107 family and is capable of oxidizing the methylene group at position 3 of &#x3b1;-guaiene to the carbonyl group.

Method of producing (-)-rotundone

The present invention aims to provide a method for efficiently producing hydroxy-L-lysine. The present invention provides a method for producing hydroxy-L-lysine, the method comprising allowing 2-oxoglutarate-dependent L-lysine hydroxylase, a cell containing 2-oxoglutarate-dependent L-lysine hydroxylase, a processed product of the cell, and/or a culture broth obtained by culturing the cell, to act on L-lysine to produce hydroxy-L-lysine represented by the following General Formula (I) (wherein each of R, Rand Rrepresents a hydrogen atom or hydroxyl group, with the proviso that at least one of R, Rand Rrepresents a hydroxyl group). 

L-lysine hydroxylase and production method for hydroxy-L-lysine and hydroxy-L-pipecolic acid using same

The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.

Process for the production of dipeptides by a dipeptide-synthesizing enzyme

Development of a method of economically and efficiently producing cis-4-hydroxy-L-proline. The present invention provides L-proline cis-4-hydroxylase. This enzyme may be derived fromor. The present invention provides a method of producing cis-4-hydroxy-L-proline from L-proline by using this enzyme. The present invention provides a recombinant vector containing a polynucleotide encoding the enzyme and a transformant containing the vector.

Process for production of cis-4-hydroxy-L-proline

L-proline cis-4-hydroxylase and use thereof to produce cis-4-hydroxy-L-proline

The present invention provides a protein having dipeptide-synthesizing activity, a DNA encoding the protein, a recombinant DNA containing the DNA, a transformant obtained via transformation with the recombinant DNA, a process for producing a protein having dipeptide-synthesizing activity using the transformant, a process for producing a dipeptide using a protein having dipeptide-synthesizing activity, and a process for producing a dipeptide using a culture or the like of a transformant or a microorganism that produces a protein having dipeptide-synthesizing activity as an enzyme source.

DNA encoding proteins having dipeptide-synthesizing activity and methods of using the same

The present invention provides a method for producing an amino acid selected from the group consisting of L-alanine, L-valine, L-leucine, L-isoleucine, L-methionine, L-phenylalanine, L-proline, glycine, L-serine, L-threonine, L-cysteine, L-tyrosine, L-asparagine, L-glutamine, L-lysine, L-histidine, L-arginine, L-aspartic acid and L-glutamic acid and useful as medicament, chemical agent, food material and feed additive at high industrial efficiency, the method comprising culturing a microorganism having an ability to produce the amino acid and having resistance to an aminoquinoline derivative in a medium, producing and accumulating the amino acid in the present invention in the culture, and recovering the amino acid from the culture.

Isolated microorganism having an ability to produce L-histidine

Provided is a novel comprising culturing host cells into which a foreign gene has been introduced for producing a protein in a soluble form encoded by the foreign gene, recombinant cells are cultured in the temperature range lower than or equal to the upper temperature limit for growth of the host cells and higher than 5 degrees below the upper temperature limit for growth of the host cells after induction of expression.

Method for protein production

A temperature-sensitive plasmid which is capable of autonomous replication inK-12 at 10&#x2013;30&#xb0; C., but, at a temperature of 33&#xb0; C. or more, is incapable of autonomous replication inK-12 or is distributed unhomogeneously upon the cell division ofK-12, thereby not to be stably carried within cells ofK-12 under said temperature, and which is incapable of autonomous replication in a microorganism belonging to the genusother thanK-12 or is distributed unhomogeneously upon cell division of said microorganism at any temperature, thereby not to be stably carried within cells of said microorganism.

[object Object]

The present invention provides an industrially efficient method for producing an L-amino acid useful as medicament, chemical agent, food material and feed additive, and the method comprising culturing in a medium a microorganism having an ability to produce the L-amino acid and having resistance to a DNA gyrase inhibitor or a microorganism having an ability to produce the L-amino acid and having both resistance to a DNA gyrase inhibitor and resistance to an aminoquinoline derivative, producing and accumulating the L-amino acid therein and recovering the L-amino acid therefrom.

Method for producing L-amino acids by fermentation

The present invention provides a process for producing an L-amino acid which comprises culturing in a nutrient medium a microorganism which is capable of producing the L-amino acid and which can not grow in a synthetic medium containing said L-amino acid as the sole nitrogen source in an amount of 5 mg/ml or below, allowing the L-amino acid to accumulate in the culture, and recovering the L-amino acid from the culture.

Process for producing L-amino acids by fermentation

Growing community of inventors

Chiba, Japan

Kuniki Kino