Jeol Ltd.

Research Development Corporation of Japan

Nagayama IP Holdings, LLC

Other

Inter-university Research Institute Corporation National Institute of Natural Sciences

Inter-university Research Institute Corporation National Institutes of Natural Sciences

Kuniaki Nagayama

Antony Stanckev Dimitrov

Hideyuki Yoshimura

Yoshihiro Arai

Toshio Yamazaki

Fumio Hosokawa

Susumu Terakawa

Hirofumi Iijima

Masanori Kataoka

Ivan B Ivanov

Radostin S Danev

Tzetzo D Douchkin

Nikolai D Denkov

Eiki Adachi

Mutsuo Matsumoto

A compound microscope device allows simultaneous observation of one specimen by a transmission electron microscope and an optical microscope. The compound microscope deviceof the present invention has a transmission electron microscopeand an optical microscope. A specimenand a reflection mirrorare disposed on an electron optical axis C of an electron ray. The reflection mirroris inclined from the electron optical axis C toward the optical object lensand the specimen. Light from the specimen(fluorescent light, reflection light, and the like) is reflected by the reflection mirrorand enters into the optical object lens. The electron ray from the electron microscopepasses through a mounting center holeof the reflection mirror. This makes it possible to observe one specimen simultaneously by the electron microscopeand the optical microscope

Compound microscope device

A method for modifying nucleic acid bases by a chemical means, which enables the discrimination of every base species in plural species of bases in a nucleic acid comprising plural nucleotide units, while retaining the base sequence information of the nucleic acid. A nucleic acid base-modified product provided by the method. The nucleic acid base-modified product is essentially a single strand. In accordance with the invention, a novel means for sequencing a nucleic acid by a microscopic means is provided.

Method for modifying nucleic acid bases, and nucleic acid base-modified product

A phase plate for an electron microscope in which a portion of a magnetic thin-wire ring or a magnetic thin-wire rod spans an opening of a support member having the opening, the magnetic thin-wire ring or magnetic thin-wire rod generates a vector potential, and a phase difference is formed between electron beams that pass through left and right sides of a spanning portion of the magnetic thin-wire ring or the magnetic thin-wire rod. The phase plate prevents the electron beam loss more effectively, can be applied at an accelerating voltage within a wide rage from a low voltage to a high voltage, causes no difficulties in production, has good utility, and makes it possible to obtain a high-contrast image.

Phase plate for electron microscope and method for manufacturing same

A method of printing an image of a three-dimensional object includes printing multiple dots of a colored ink to form an image of the object and printing dots of a transparent ink having a refractive index greater than 1 within the image. The dots of transparent ink local alter the path length of light that shines through the image to create phase variations on the image.

Complex pointillistic printing

A method for determining the sequence of at least a portion of a single-stranded nucleic acid molecule by base-specifically labeling the exposed bases of the nucleic acid molecule using heavy element-substituted nucleotide bases which form Watson-Crick type base-pairs with the exposed bases of the nucleic acid molecule and then imaging the labeled single-stranded nucleic acid molecule using electron microscopy, e.g., transmission electron microscopy (TEM), or some other method that permits discrimination of the heavy element substituted nucleotide bases is described. The image is analyzed to determine the base sequence of at least a portion of the nucleic acid molecule.

Method for nucleic acid sequencing

Novel DNA sequence determination method and DNA sequencer system providing a sequencing speed 10to 10times faster than the current DNA sequencing speed (10bases per day with a lane at maximum) of the existing DNA sequencer based on electrophoresis. The method includes the step of discriminating base-specific labels of heavy elements using a magnified image of elongated single-chain DNA or RNA produced by a transmission electron microscope (TEM). The DNA sequencer system uses this method. The invention provides a DNA sequencing speed that is higher than the existing speed by 3 or 4 orders of magnitude.

Method of determining base sequence of DNA or RNA using heavy element labeling and imaging by transmission electron microscopy

A method of forming a particle film on a surface of a solid or liquid substrate involves contacting the substrate, in the presence of a gas, with a liquid medium containing a plurality of particles suspended therein. A liquid meniscus is thereby formed between the substrate and the gas. An edge of the liquid meniscus is moved relative to the substrate, so that said particles in the liquid medium form the particle film on the surface of the substrate.

Method for producing a continuous, large-area particle film

A lens system for use with a phase plate in a transmission electron microscope comprises a phase plate placed after the back-focal plane of the objective lens in an imaging system mounted downstream of the objective lens. Phase lenses image the back-focal plane of the objective lens onto the phase plate such that the position and tilt of the electron beam relative to the optical axis are made conjugate. An alignment coil may direct the electron beam going out of the phase lenses toward the phase plate. A second alignment coil may direct the electron beam going out of the phase plate toward the imaging lenses located after the phase plate.

Lens system for phase plate for transmission electron microscope and transmission electron microscope

Phase manipulation is used to produce a high contrast electron microscope image. A phase plate is placed at the back focal plane of an objective lens and used to form a differential contrast image.

Differential contrast transmission electron microscope and method of processing data about electron microscope images

The present invention provides a high-luminance opal-like diffraction chromogenic film having no dead angle, which comprises a hexagonally close-packed grating monolayer film of micron-order particulates.

Chromogenic film having a diffraction pattern similar to an opal

Protein is two-dimensionally aggregated and fixed to produce a functional thin film. A denatured film of a first protein is formed on the surface of a substrate solution such as an aqueous sugar or salt solution. A solution of a second protein which may be the same as or different from the first protein is then injected below the surface of the substrate solution at a distance sufficient not to disturb the surface. The substrate solution has a higher surface tension and specific gravity than the second protein solution causing the second protein to float between the denatured protein film and the substrate solution surface to form the two-dimensionally aggregated and fixed protein. The denatured film of first protein may be formed by injection of a solution of the first protein below the surface of the substrate solution. The aggregated and fixed protein can be heated and then transferred onto a solid surface such as in the formation of a biosensor. A three-dimensional multilayer laminate of the two-dimensional aggregate may also be formed. In an alternative embodiment, a lipid monolayer is formed on the surface of the substrate solution, a protein solution is injected below the surface of the substrate solution and the protein floats between the substrate solution surface and the lipid monolayer to form the two-dimensionally aggregated and fixed protein.

Two-dimensional aggregation and fixation of protein by injection into a

To observe film thickness and/or refractive index of a sample, light beams from a white light source are irradiated onto the surface of the solid or liquid sample such that the reflected light beams are colored by partial extinction of the reflected light beams. A difference 17 film thickness and/or refractive index of the sample is observed according to color difference of the reflected light beams.

Method to observe film thickness and/or refractive index by color

A method of supplying a liquid dispersive medium of fine particles onto the surface of a substrate having a flat surface, forming a liquid thin film and controlling the liquid thickness of the liquid dispersive medium through evaporation, for example, thereby assembling the particles in a two-dimensional manner. By controlling the liquid thickness of the liquid dispersive medium of the particles, it is possible to cause rapid two-dimensional assembly of the particles, and in addition, to control the order of particle size in the assembly. Fixing of the assembly permits the formation of a film structure.

Method for two-dimensional assembly formation of fine particles from a

A particle-containing or particle-forming liquid is spread over the surface of a high density liquid; the particles are aggregated two-dimensionally by reducing the thickness of the particle-containing or particle-forming liquid which has been spread; and the aggregated and formed two-dimensional particle thin film is brought into contact with the surface of a solid substrate to transfer and fix said thin film thereupon. By this method, it is possible to form a two-dimensional aggregate quickly, and form two-dimensional particle thin film of a high quality.

Method for forming a two-dimensional thin film of particles

Method and apparatus for forming a two-dimensional assembly of fine particles on a surface. A wall cell is placed in contact with or near the surface of a solid substrate so as to form a closed surface region on the surface of the solid substrate, a liquid containing fine particles is injected onto the closed surface region in the wall cell, and then the liquid is removed at a controlled rate, thereby causing a two-dimensional assembly of fine particles to form on the solid surface.

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Aichi, Japan

Kuniaki Nagayama